PMID- 15525597
OWN - NLM
STAT- MEDLINE
DCOM- 20050426
LR  - 20191210
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 33
IP  - 2
DP  - 2004 Oct
TI  - Estrogen response element-dependent regulation of transcriptional activation of 
      estrogen receptors alpha and beta by coactivators and corepressors.
PG  - 387-410
AB  - One mechanism by which ligand-activated estrogen receptors alpha and beta 
      (ERalpha and ERbeta) stimulate gene transcription is through direct ER 
      interaction with specific DNA sequences, estrogen response elements (EREs). 
      ERE-bound ER recruits coactivators that stimulate gene transcription. Binding of 
      ER to natural and synthetic EREs with different nucleotide sequences alters ER 
      binding affinity, conformation, and transcriptional activity, indicating that the 
      ERE sequence is an allosteric effector of ER action. Here we tested the 
      hypothesis that alterations in ER conformation induced by binding to different 
      ERE sequences modulates ER interaction with coactivators and corepressors. CHO-K1 
      cells transfected with ERalpha or ERbeta show ERE sequence-dependent differences 
      in the functional interaction of ERalpha and ERbeta with coactivators steroid 
      receptor coactivator 1 (SRC-1), SRC-2 (glucocorticoid receptor interacting 
      protein 1 (GRIP1)), SRC-3 amplified in breast cancer 1 (AIB1) and ACTR, cyclic 
      AMP binding protein (CBP), and steroid receptor RNA activator (SRA), corepressors 
      nuclear receptor co-repressor (NCoR) and silencing mediator for retinoid and 
      thyroid hormone receptors (SMRT), and secondary coactivators coactivator 
      associated arginine methyltransferase 1 (CARM1) and protein arginine 
      methyltransferase 1 (PRMT1). We note both ligand-independent as well estradiol- 
      and 4-hydroxytamoxifen-dependent differences in ER-coregulator activity. In vitro 
      ER-ERE binding assays using receptor interaction domains of these coregulators 
      failed to recapitulate the cell-based results, substantiating the importance of 
      the full-length proteins in regulating ER activity. These data demonstrated that 
      the ERE sequence impacts estradiol-and 4-hydroxytamoxifen-occupied ERalpha and 
      ERbeta interaction with coregulators as measured by transcriptional activity in 
      mammalian cells.
FAU - Klinge, C M
AU  - Klinge CM
AD  - Department of Biochemistry and Molecular Biology, Center for Genetics and 
      Molecular Medicine, University of Louisville School of Medicine, Louisville, 
      Kentucky 40292, USA. carolyn.klinge@louisville.edu
FAU - Jernigan, S C
AU  - Jernigan SC
FAU - Mattingly, K A
AU  - Mattingly KA
FAU - Risinger, K E
AU  - Risinger KE
FAU - Zhang, J
AU  - Zhang J
LA  - eng
GR  - R01 DK 53220/DK/NIDDK NIH HHS/United States
GR  - T35 GM08561/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (NCOA2 protein, human)
RN  - 0 (NCOA3 protein, Xenopus)
RN  - 0 (NCOR1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nuclear Receptor Co-Repressor 1)
RN  - 0 (Nuclear Receptor Coactivator 2)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Repressor Proteins)
RN  - 0 (Selective Estrogen Receptor Modulators)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Xenopus Proteins)
RN  - 0 (steroid receptor RNA activator)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 17197F0KYM (afimoxifene)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.1.1.319 (Protein-Arginine N-Methyltransferases)
RN  - EC 2.1.1.319 (coactivator-associated arginine methyltransferase 1)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (CREBBP protein, human)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - CREB-Binding Protein
MH  - Cricetinae
MH  - Cricetulus
MH  - Estradiol/metabolism/pharmacology
MH  - Estrogen Receptor alpha/drug effects/genetics/*metabolism
MH  - Estrogen Receptor beta/drug effects/genetics/*metabolism
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Nuclear Receptor Co-Repressor 1
MH  - Nuclear Receptor Coactivator 1
MH  - Nuclear Receptor Coactivator 2
MH  - Nuclear Receptor Coactivator 3
MH  - Protein-Arginine N-Methyltransferases/metabolism
MH  - RNA, Long Noncoding
MH  - RNA, Untranslated/metabolism
MH  - Repressor Proteins/metabolism
MH  - *Response Elements/drug effects/genetics
MH  - Selective Estrogen Receptor Modulators/pharmacology
MH  - Tamoxifen/*analogs & derivatives/pharmacology
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/metabolism
MH  - Transcriptional Activation
MH  - Xenopus Proteins/metabolism
EDAT- 2004/11/05 09:00
MHDA- 2005/04/27 09:00
CRDT- 2004/11/05 09:00
PHST- 2004/11/05 09:00 [pubmed]
PHST- 2005/04/27 09:00 [medline]
PHST- 2004/11/05 09:00 [entrez]
AID - 33/2/387 [pii]
AID - 10.1677/jme.1.01541 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 2004 Oct;33(2):387-410. doi: 10.1677/jme.1.01541.