PMID- 15525761
OWN - NLM
STAT- MEDLINE
DCOM- 20050609
LR  - 20220309
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 24
IP  - 44
DP  - 2004 Nov 3
TI  - Brain-derived neurotrophic factor induces mammalian target of rapamycin-dependent 
      local activation of translation machinery and protein synthesis in neuronal 
      dendrites.
PG  - 9760-9
AB  - In neurons, perisynaptic or dendritic translation is implicated in synapse-wide 
      alterations of function and morphology triggered by neural activity. The 
      molecular mechanisms controlling local translation activation, however, have yet 
      to be elucidated. Here, we show that local protein synthesis and translational 
      activation in neuronal dendrites are upregulated by brain-derived neurotrophic 
      factor (BDNF) in a rapamycin and small interfering RNA specific for mammalian 
      target of rapamycin (mTOR)-sensitive manner. In parallel, BDNF induced the 
      phosphorylation of tuberin and the activation of mTOR in dendrites and the 
      synaptoneurosome fraction. mTOR activation stimulated translation initiation 
      processes involving both eIF4E/4E-binding protein (4EBP) and p70S6 
      kinase/ribosomal S6 protein. BDNF induced phosphorylation of 4EBP in isolated 
      dendrites. Moreover, local puff application of BDNF to dendrites triggered S6 
      phosphorylation in a restricted area. Taken together, these data indicate that 
      mTOR-dependent translation activation is essential for the upregulation of local 
      protein synthesis in neuronal dendrites.
FAU - Takei, Nobuyuki
AU  - Takei N
AD  - Department of Molecular Neurobiology, Brain Research Institute, Niigata 
      University, Niigata 951-8585, Japan. nobtak@bri.niigata-u.ac.jp
FAU - Inamura, Naoko
AU  - Inamura N
FAU - Kawamura, Mihoko
AU  - Kawamura M
FAU - Namba, Hisaaki
AU  - Namba H
FAU - Hara, Kenta
AU  - Hara K
FAU - Yonezawa, Kazuyoshi
AU  - Yonezawa K
FAU - Nawa, Hiroyuki
AU  - Nawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Ras Homolog Enriched in Brain Protein)
RN  - 0 (Repressor Proteins)
RN  - 0 (Rheb protein, rat)
RN  - 0 (Tuberous Sclerosis Complex 2 Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/metabolism
MH  - Dendrites/drug effects/*metabolism
MH  - Gene Expression Regulation
MH  - Monomeric GTP-Binding Proteins/analysis
MH  - Nerve Tissue Proteins/analysis/*biosynthesis
MH  - Neuronal Plasticity/physiology
MH  - Neuropeptides/analysis
MH  - Protein Biosynthesis/drug effects/physiology
MH  - Protein Kinases/analysis/*physiology
MH  - RNA, Small Interfering
MH  - Ras Homolog Enriched in Brain Protein
MH  - Rats
MH  - Repressor Proteins/analysis
MH  - Sirolimus/pharmacology
MH  - Synaptosomes/metabolism
MH  - TOR Serine-Threonine Kinases
MH  - Tuberous Sclerosis Complex 2 Protein
MH  - Tumor Suppressor Proteins/analysis
PMC - PMC6730227
EDAT- 2004/11/05 09:00
MHDA- 2005/06/10 09:00
PMCR- 2005/05/03
CRDT- 2004/11/05 09:00
PHST- 2004/11/05 09:00 [pubmed]
PHST- 2005/06/10 09:00 [medline]
PHST- 2004/11/05 09:00 [entrez]
PHST- 2005/05/03 00:00 [pmc-release]
AID - 24/44/9760 [pii]
AID - 10.1523/JNEUROSCI.1427-04.2004 [doi]
PST - ppublish
SO  - J Neurosci. 2004 Nov 3;24(44):9760-9. doi: 10.1523/JNEUROSCI.1427-04.2004.