PMID- 15526196
OWN - NLM
STAT- MEDLINE
DCOM- 20050630
LR  - 20131121
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 66
IP  - 5
DP  - 2005 Feb
TI  - Klebsiella planticola strain DSZ mineralizes simazine: physiological adaptations 
      involved in the process.
PG  - 589-96
AB  - We examined the ability of a soil bacterium, Klebsiella planticola strain DSZ, to 
      degrade the herbicide simazine (SZ). Strain DSZ is metabolically diverse and 
      grows on a wide range of s-triazine and aromatic compounds. DSZ cells grown in 
      liquid medium with SZ (in 10 mM ethanol) as carbon source mineralized 71.6+/-1.3% 
      of 0.025 mM SZ with a yield of 4.6+/-0.3 microg cell dry weight mmol(-1) carbon. 
      The metabolites produced by DSZ during SZ degradation included ammeline, cyanuric 
      acid, N-formylurea and urea. We studied the physiological adaptations which allow 
      strain DSZ to metabolize SZ. Using scanning electron microscopy, we detected DSZ 
      cells covering the surfaces of SZ crystals when the herbicide was used at high 
      concentrations (0.1 mM). The membrane order observed by FTIR spectroscopy showed 
      membrane activity at low temperature (4 degrees C) to assimilate the herbicide. 
      Membrane fatty acid analysis demonstrated that strain DSZ adapted to grow on SZ 
      by increasing the degree of saturation of membrane lipid fatty acid; and the 
      opposite effect was detected when both SZ and ethanol were used as carbon 
      sources. This confirms the modulator effect of ethanol on membrane fluidity.
FAU - Sanchez, Mariela
AU  - Sanchez M
AD  - Departamento de Bioquimica y Biologia Molecular IV, Universidad Complutense, 
      Madrid, Spain.
FAU - Garbi, Carlos
AU  - Garbi C
FAU - Martinez-Alvarez, Roberto
AU  - Martinez-Alvarez R
FAU - Ortiz, Luis T
AU  - Ortiz LT
FAU - Allende, Jose Luis
AU  - Allende JL
FAU - Martin, Margarita
AU  - Martin M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041104
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Fatty Acids)
RN  - 0 (Herbicides)
RN  - 0 (Minerals)
RN  - 0 (Soil Pollutants)
RN  - 3K9958V90M (Ethanol)
RN  - SG0C34SMY3 (Simazine)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Biodegradation, Environmental
MH  - Cell Membrane/metabolism
MH  - Cold Temperature
MH  - Ethanol/metabolism
MH  - Fatty Acids/chemistry/*metabolism
MH  - Herbicides/metabolism
MH  - Klebsiella/classification/metabolism/*physiology/ultrastructure
MH  - Minerals/*metabolism
MH  - Simazine/*metabolism
MH  - Soil Pollutants/*metabolism/toxicity
MH  - Temperature
EDAT- 2004/11/05 09:00
MHDA- 2005/07/01 09:00
CRDT- 2004/11/05 09:00
PHST- 2004/05/20 00:00 [received]
PHST- 2004/07/29 00:00 [accepted]
PHST- 2004/07/27 00:00 [revised]
PHST- 2004/11/05 09:00 [pubmed]
PHST- 2005/07/01 09:00 [medline]
PHST- 2004/11/05 09:00 [entrez]
AID - 10.1007/s00253-004-1735-y [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2005 Feb;66(5):589-96. doi: 10.1007/s00253-004-1735-y. 
      Epub 2004 Nov 4.