PMID- 1552742
OWN - NLM
STAT- MEDLINE
DCOM- 19920424
LR  - 20131121
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 6
IP  - 2
DP  - 1992 Feb
TI  - Production of tumor necrosis factor-alpha by normal and malignant B lymphocytes 
      in response to interferon-alpha, interferon-gamma and interleukin-4.
PG  - 116-9
AB  - Since autocrine stimulation by tumor necrosis factor-alpha (TNF alpha) may be 
      implicated in the proliferation of normal and malignant B cells, we measured the 
      production of TNF alpha protein by these cells in response to various B-cell 
      stimulatory agents. Purified malignant and non-malignant B lymphocytes were 
      incubated with interleukin-4 (IL4), interferon-alpha (IFN alpha) and IFN gamma, 
      and the supernatants were tested for the production of TNF alpha using an 
      enzyme-linked immunosorbent assay (ELISA). Chronic lymphocytic (CLL) and 
      prolymphocytic (PLL) leukemia cells produced low amounts of TNF alpha, 
      irrespectively of the addition of inducers. Normal B lymphocytes (tonsillar and 
      blood) produced TNF alpha, but the level was not influenced by any of the 
      inducers tested. Hairy cell leukemia (HCL) cells produced TNF alpha in the 
      absence of stimuli and this production was markedly enhanced by addition of IFN 
      alpha or, to a lesser extent, by IFN gamma and IL4. These results contradict the 
      hypothesis that IFN alpha exerts its therapeutic action in HCL by inhibition of 
      autocrine TNF alpha production.
FAU - Jansen, J H
AU  - Jansen JH
AD  - Department of Hematology, University Medical Center, Leiden, The Netherlands.
FAU - Wientjens, G J
AU  - Wientjens GJ
FAU - Willemze, R
AU  - Willemze R
FAU - Kluin-Nelemans, J C
AU  - Kluin-Nelemans JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Interferon-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
CIN - Leukemia. 1993 Feb;7(2):331-2. PMID: 8426486
MH  - B-Lymphocytes/drug effects/*metabolism
MH  - Cycloheximide/pharmacology
MH  - Humans
MH  - Interferon-alpha/*pharmacology
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Leukemia, Hairy Cell/blood
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/blood
MH  - Leukemia, Prolymphocytic/blood
MH  - Palatine Tonsil/cytology
MH  - Transcription, Genetic
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1992 Feb;6(2):116-9.