PMID- 15528542
OWN - NLM
STAT- MEDLINE
DCOM- 20050228
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 70
IP  - 11
DP  - 2004 Nov
TI  - Changes in bacterioplankton composition under different phytoplankton regimens.
PG  - 6753-66
AB  - The results of empirical studies have revealed links between phytoplankton and 
      bacterioplankton, such as the frequent correlation between chlorophyll a and bulk 
      bacterial abundance and production. Nevertheless, little is known about possible 
      links at the level of specific taxonomic groups. To investigate this issue, 
      seawater microcosm experiments were performed in the northwestern Mediterranean 
      Sea. Turbulence was used as a noninvasive means to induce phytoplankton blooms 
      dominated by different algae. Microcosms exposed to turbulence became dominated 
      by diatoms, while small phytoflagellates gained importance under still 
      conditions. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene 
      fragments showed that changes in phytoplankton community composition were 
      followed by shifts in bacterioplankton community composition, both as changes in 
      the presence or absence of distinct bacterial phylotypes and as differences in 
      the relative abundance of ubiquitous phylotypes. Sequencing of DGGE bands showed 
      that four Roseobacter phylotypes were present in all microcosms. The microcosms 
      with a higher proportion of phytoflagellates were characterized by four 
      phylotypes of the Bacteroidetes phylum: two affiliated with the family 
      Cryomorphaceae and two with the family Flavobacteriaceae. Two other 
      Flavobacteriaceae phylotypes were characteristic of the diatom-dominated 
      microcosms, together with one Alphaproteobacteria phylotype (Roseobacter) and one 
      Gammaproteobacteria phylotype (Methylophaga). Phylogenetic analyses of published 
      Bacteroidetes 16S rRNA gene sequences confirmed that members of the 
      Flavobacteriaceae are remarkably responsive to phytoplankton blooms, indicating 
      these bacteria could be particularly important in the processing of organic 
      matter during such events. Our data suggest that quantitative and qualitative 
      differences in phytoplankton species composition may lead to pronounced 
      differences in bacterioplankton species composition.
FAU - Pinhassi, Jarone
AU  - Pinhassi J
AD  - Institut de Ciencies del Mar-CMIMA (CSIC), Barcelona, Catalunya, Spain. 
      jarone.pinhassi@hik.se
FAU - Sala, Maria Montserrat
AU  - Sala MM
FAU - Havskum, Harry
AU  - Havskum H
FAU - Peters, Francesc
AU  - Peters F
FAU - Guadayol, Oscar
AU  - Guadayol O
FAU - Malits, Andrea
AU  - Malits A
FAU - Marrase, Celia
AU  - Marrase C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/genetics/growth & development
MH  - Bacteroidetes/classification/genetics/growth & development
MH  - Diatoms/growth & development
MH  - Dinoflagellida/growth & development
MH  - *Ecosystem
MH  - Electrophoresis/methods
MH  - Eutrophication/*physiology
MH  - Gammaproteobacteria/classification/genetics/growth & development
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Phytoplankton/*classification/genetics/growth & development
MH  - Plankton/*classification/genetics/growth & development
MH  - RNA, Ribosomal, 16S/genetics
MH  - Roseobacter/classification/genetics/growth & development
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
PMC - PMC525254
EDAT- 2004/11/06 09:00
MHDA- 2005/03/01 09:00
PMCR- 2004/11/01
CRDT- 2004/11/06 09:00
PHST- 2004/11/06 09:00 [pubmed]
PHST- 2005/03/01 09:00 [medline]
PHST- 2004/11/06 09:00 [entrez]
PHST- 2004/11/01 00:00 [pmc-release]
AID - 70/11/6753 [pii]
AID - 1018-04 [pii]
AID - 10.1128/AEM.70.11.6753-6766.2004 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2004 Nov;70(11):6753-66. doi: 
      10.1128/AEM.70.11.6753-6766.2004.