PMID- 15528691
OWN - NLM
STAT- MEDLINE
DCOM- 20050127
LR  - 20191210
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 42
IP  - 11
DP  - 2004 Nov
TI  - Molecular characteristics of strains of the cameroon family, the major group of 
      Mycobacterium tuberculosis in a country with a high prevalence of tuberculosis.
PG  - 5029-35
AB  - A preliminary investigation of the genetic biodiversity of Mycobacterium 
      tuberculosis complex strains in Cameroon, a country with a high prevalence of 
      tuberculosis, described a group of closely related M. tuberculosis strains (the 
      Cameroon family) currently responsible for more than 40% of smear-positive 
      pulmonary tuberculosis cases. Here, we used various molecular methods to study 
      the genetic characteristics of this family of strains. Cameroon family M. 
      tuberculosis strains (i) are part of the major genetic group 2 and lack the TbD1 
      region like other families of epidemic strains, (ii) lack spacers 23, 24, and 25 
      in their direct repeat (DR) region, (iii) have an identical number of repeats in 
      8 of 12 variable-number tandem repeats of mycobacterial interspersed repetitive 
      unit (MIRU-VNTR) loci, (iv) have similar IS6110-restriction fragment length 
      polymorphism (RFLP) multiband patterns (10 to 15 copies) with seven common IS6110 
      bands, (v) do not have an IS6110 element in their DR locus, and (vi) have four 
      IS6110 elements in open reading frames (adenylate cyclase, phospholipase C, moeY, 
      and ATP binding genes). Analysis by spoligotyping, MIRU-VNTR, and IS6110-RFLP 
      typing methods revealed differences not observed in previous studies; 
      polymorphism as assessed by MIRU-VNTR typing was lower than suggested by 
      spoligotyping, and in rare cases, strains with identical IS6110-RFLP patterns had 
      spoligotypes differing by as much as 15 spacers. Our findings confirm the recent 
      expansion of this family in Cameroon and indicate that the interpretation of 
      molecular typing results has to be adapted to the characteristics of the strain 
      population within each setting. The knowledge of this particular genotype, with 
      its large involvement in tuberculosis in Cameroon, allows greater refinement of 
      tuberculosis transmission studies by interpreting data in the context of this 
      geographic area.
FAU - Niobe-Eyangoh, Sara Ngo
AU  - Niobe-Eyangoh SN
AD  - Centre National de Reference des Mycobacteries, Institut Pasteur, 25 rue du Dr 
      Roux, 75724 Cedex 15, Paris, France.
FAU - Kuaban, Christopher
AU  - Kuaban C
FAU - Sorlin, Philippe
AU  - Sorlin P
FAU - Thonnon, Jocelyn
AU  - Thonnon J
FAU - Vincent, Veronique
AU  - Vincent V
FAU - Gutierrez, M Cristina
AU  - Gutierrez MC
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Genetic Markers)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Bacterial Proteins/genetics
MH  - *Bacterial Typing Techniques
MH  - Cameroon/epidemiology
MH  - DNA Transposable Elements
MH  - Genetic Markers/genetics
MH  - Humans
MH  - Minisatellite Repeats/genetics
MH  - Mutagenesis, Insertional
MH  - Mycobacterium tuberculosis/*classification/*genetics
MH  - Oligonucleotides/analysis
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Prevalence
MH  - Tuberculosis, Pulmonary/*epidemiology/microbiology
PMC - PMC525220
EDAT- 2004/11/06 09:00
MHDA- 2005/01/28 09:00
PMCR- 2004/11/01
CRDT- 2004/11/06 09:00
PHST- 2004/11/06 09:00 [pubmed]
PHST- 2005/01/28 09:00 [medline]
PHST- 2004/11/06 09:00 [entrez]
PHST- 2004/11/01 00:00 [pmc-release]
AID - 42/11/5029 [pii]
AID - 0658-04 [pii]
AID - 10.1128/JCM.42.11.5029-5035.2004 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2004 Nov;42(11):5029-35. doi: 10.1128/JCM.42.11.5029-5035.2004.