PMID- 15529267
OWN - NLM
STAT- MEDLINE
DCOM- 20041230
LR  - 20061115
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 190
IP  - 11
DP  - 2004 Dec 1
TI  - Antibodies directed against O-acetylated sialoglycoconjugates accelerate 
      complement activation in Leishmania donovani promastigotes.
PG  - 2010-9
AB  - BACKGROUND: An enhanced presence of 9-O-acetylated sialoglycoconjugates 
      (9-O-AcSGs) triggers the alternate pathway (AP) in Indian visceral leishmaniasis 
      (VL). Antibodies directed against these epitopes are present in high titers. The 
      biological relevance of these antibodies, with regard to activation of the 
      classical pathway (CP), was investigated. METHODS: Complement activators were 
      affinity purified, complement activation via the CP, AP, and lectin-mediated 
      complement pathway was measured by use of an anti-C3 radio-binding assay, and the 
      number of C3 molecules was quantitated by Scatchard analysis. Cell death induced 
      via the complement pathways was measured by use of MTT (tetrazolium salt 3- [4, 
      5-dimethylthiazol-2-yl] -2, 5-diphenyltetrazolium bromide) assay, and uptake of 
      propidium iodide (PI) was measured by flow cytometry. RESULTS: Anti-O-AcSGs from 
      both healthy donors and patients with VL elicited C3 deposition as early as 3 
      min, which triggered parasite lysis, as demonstrated by use of MTT assay and 
      corroborated by the high rate of uptake of PI. Analysis of complement activation 
      by mannan-binding lectin and C-reactive protein demonstrated their negligible 
      contribution during the 3-min time frame. CONCLUSIONS: Anti-O-AcSGs were 
      identified as an important source of CP activation under normal physiological 
      conditions, suggesting that they play a role in conferring host protection 
      against parasite infection.
FAU - Bandyopadhyay, Sumi
AU  - Bandyopadhyay S
AD  - Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick 
      Road, Jadavpur, Kolkata 700-032, India.
FAU - Chatterjee, Mitali
AU  - Chatterjee M
FAU - Das, Tanusree
AU  - Das T
FAU - Bandyopadhyay, Suman
AU  - Bandyopadhyay S
FAU - Sundar, Shyam
AU  - Sundar S
FAU - Mandal, Chitra
AU  - Mandal C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041103
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Complement C3)
RN  - 0 (Glycoconjugates)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/isolation & purification/*pharmacology
MH  - Complement Activation/*drug effects
MH  - Complement C3/analysis/*immunology
MH  - Complement Pathway, Alternative/drug effects
MH  - Complement Pathway, Classical/drug effects
MH  - Dose-Response Relationship, Immunologic
MH  - Glycoconjugates/*immunology
MH  - Humans
MH  - Leishmania donovani/drug effects/*immunology
MH  - Leishmaniasis, Visceral/blood/*immunology
MH  - Time Factors
EDAT- 2004/11/06 09:00
MHDA- 2004/12/31 09:00
CRDT- 2004/11/06 09:00
PHST- 2004/03/17 00:00 [received]
PHST- 2004/06/14 00:00 [accepted]
PHST- 2004/11/06 09:00 [pubmed]
PHST- 2004/12/31 09:00 [medline]
PHST- 2004/11/06 09:00 [entrez]
AID - JID32710 [pii]
AID - 10.1086/425519 [doi]
PST - ppublish
SO  - J Infect Dis. 2004 Dec 1;190(11):2010-9. doi: 10.1086/425519. Epub 2004 Nov 3.