PMID- 15530414
OWN - NLM
STAT- MEDLINE
DCOM- 20050217
LR  - 20181201
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 325
IP  - 2
DP  - 2004 Dec 10
TI  - Cartilage-derived morphogenetic protein-1 promotes the differentiation of 
      mesenchymal stem cells into chondrocytes.
PG  - 453-60
AB  - Mesenchymal stem cells (MSCs) are able to differentiate into many types of cells 
      including chondrocytes. Transforming growth factor beta1 (TGF-beta1) is very 
      important in the regulation of chondrogenesis. Since cartilage-derived 
      morphogenetic protein-1 (CDMP-1) belongs to the TGF-beta superfamily, we tested 
      whether CDMP-1 plays any role in the regulation of the differentiation of MSCs 
      into chondrocytes using a high density pellet culture system. Based on the 
      histological staining of glycosaminoglycan using toluidine blue dye-binding 
      method we found that CDMP-1 could initiate chondrogenic differentiation of MSCs 
      as did TGF-beta1. However, CDMP-1 was less stimulatory than TGF-beta1. The 
      combination of CDMP-1 and TGF-beta1 synergically induced chondrogenesis of MSCs. 
      This synergic chondrogenic effect of CDMP-1 together with TGF-beta1 was further 
      confirmed by quantification of GAG using dimethylmethylene blue dye-binding assay 
      and immunohistochemical analysis of the expression of cartilage-specific protein 
      collagen II. This study may provide an improved induction approach using MSCs for 
      repairing damaged cartilage.
FAU - Bai, Xiaowen
AU  - Bai X
AD  - Peking University Stem Cell Research Center, Peking University Health Science 
      Center, 38 Xue Yuan Road, Beijing 100083, China.
FAU - Xiao, Zhifeng
AU  - Xiao Z
FAU - Pan, Yuqiong
AU  - Pan Y
FAU - Hu, Jiang
AU  - Hu J
FAU - Pohl, Jens
AU  - Pohl J
FAU - Wen, Jinhua
AU  - Wen J
FAU - Li, Lingsong
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Growth Differentiation Factor 5)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Aborted Fetus/cytology
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Chondrocytes/*cytology/drug effects
MH  - Chondrogenesis/drug effects/physiology
MH  - Collagen/biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression/drug effects
MH  - Glycosaminoglycans/biosynthesis
MH  - Growth Differentiation Factor 5
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/*drug effects
MH  - Transforming Growth Factor beta/pharmacology
MH  - Transforming Growth Factor beta1
EDAT- 2004/11/09 09:00
MHDA- 2005/02/18 09:00
CRDT- 2004/11/09 09:00
PHST- 2004/09/30 00:00 [received]
PHST- 2004/11/09 09:00 [pubmed]
PHST- 2005/02/18 09:00 [medline]
PHST- 2004/11/09 09:00 [entrez]
AID - S0006-291X(04)02318-6 [pii]
AID - 10.1016/j.bbrc.2004.10.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 Dec 10;325(2):453-60. doi: 
      10.1016/j.bbrc.2004.10.055.