PMID- 15530506
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20171116
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 76
IP  - 4
DP  - 2004 Dec 10
TI  - Interaction between the polyol pathway and non-enzymatic glycation on aortic 
      smooth muscle cell migration and monocyte adhesion.
PG  - 445-59
AB  - We investigated for the interaction between the polyol pathway and enhanced 
      non-enzymatic glycation, both implicated in the pathogenesis of diabetic 
      atherosclerosis, in the activation of aortic smooth muscle cell (SMC) function. 
      Mouse aortas and primary cultures of SMCs from wildtype (WT) mice and transgenic 
      (TG) mice expressing human aldose reductase (AR) were studied regarding changes 
      in AR activity, and SMC gene activation, migration and monocyte adhesion, in 
      response to advanced glycation end-product modified BSA (AGE-BSA). Results showed 
      that AGE-BSA increased AR activity in both WT and TG aortas, with greater 
      increments (p < 0.05) in TG aortas which, basally, had elevated AR activity (2.8 
      fold of WT). These increments were attenuated by zopolrestat, an AR inhibitor. 
      Similar AGE-induced increments in AR activity were observed in primary cultures 
      of aortic SMCs from WT and TG mice (60% and 100%, respectively, P < 0.01). Such 
      increments were accompanied by increases in intercellular adhesion molecule-1 
      (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1) mRNA levels (both P < 
      0.05), activation of membrane-associated PKC-beta1 (P < 0.05) as well as 
      increased SMC migration and Tamm-Horsfall protein (THP)-1 monocyte adhesion to 
      SMCs (both p < 0.01), with all changes being significantly greater in TG SMCs (P 
      < 0.05) and suppressible by either zopolrestat or transfection with an AR 
      antisense oligonucleotide. Our findings suggest that the effects of AGEs on SMC 
      activation, migration and monocyte adhesion are mediated partly through the 
      polyol pathway and, possibly, PKC activation. The greater AGE-induced changes in 
      the TG SMCs have provided further support for the dependency of such changes on 
      polyol pathway hyperactivity.
FAU - Dan, Qinghong
AU  - Dan Q
AD  - Department of Medicine, The University of Hong Kong, Queen Mary Hospital, 102 
      Pokfulam Road, Hong Kong SAR, China.
FAU - Wong, Rachel
AU  - Wong R
FAU - Chung, Sookja K
AU  - Chung SK
FAU - Chung, Stephen S M
AU  - Chung SS
FAU - Lam, Karen S L
AU  - Lam KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Antigens, CD)
RN  - 0 (Benzothiazoles)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Hint1 protein, mouse)
RN  - 0 (ICAM-2 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligoribonucleotides, Antisense)
RN  - 0 (Phthalazines)
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Thiazoles)
RN  - 0 (advanced glycation end products-bovine serum albumin)
RN  - 0 (polyol)
RN  - 1PV3S9WP3D (zopolrestat)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - EC 1.1.1.21 (Aldehyde Reductase)
SB  - IM
MH  - Aldehyde Reductase/antagonists & inhibitors/genetics/metabolism
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Aorta, Thoracic/*drug effects/metabolism
MH  - Benzothiazoles
MH  - Blotting, Northern
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/genetics/metabolism
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Glycation End Products, Advanced/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/cytology/*drug effects
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Oligoribonucleotides, Antisense/pharmacology
MH  - Phthalazines/pharmacology
MH  - Polymers/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serum Albumin, Bovine/*pharmacology
MH  - Thiazoles/pharmacology
EDAT- 2004/11/09 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/11/09 09:00
PHST- 2004/04/30 00:00 [received]
PHST- 2004/09/15 00:00 [accepted]
PHST- 2004/11/09 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/11/09 09:00 [entrez]
AID - S0024-3205(04)00839-2 [pii]
AID - 10.1016/j.lfs.2004.09.010 [doi]
PST - ppublish
SO  - Life Sci. 2004 Dec 10;76(4):445-59. doi: 10.1016/j.lfs.2004.09.010.