PMID- 15530862
OWN - NLM
STAT- MEDLINE
DCOM- 20050103
LR  - 20211203
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 301
IP  - 2
DP  - 2004 Dec 10
TI  - Topology of genes and nontranscribed sequences in human interphase nuclei.
PG  - 266-79
AB  - Knowledge about the functional impact of the topological organization of DNA 
      sequences within interphase chromosome territories is still sparse. Of the few 
      analyzed single copy genomic DNA sequences, the majority had been found to 
      localize preferentially at the chromosome periphery or to loop out from 
      chromosome territories. By means of dual-color fluorescence in situ hybridization 
      (FISH), immunolabeling, confocal microscopy, and three-dimensional (3D) image 
      analysis, we analyzed the intraterritorial and nuclear localization of 10 genomic 
      fragments of different sequence classes in four different human cell types. The 
      localization of three muscle-specific genes FLNA, NEB, and TTN, the oncogene 
      BCL2, the tumor suppressor gene MADH4, and five putatively nontranscribed genomic 
      sequences was predominantly in the periphery of the respective chromosome 
      territories, independent from transcriptional status and from GC content. In 
      interphase nuclei, the noncoding sequences were only rarely found associated with 
      heterochromatic sites marked by the satellite III DNA D1Z1 or clusters of 
      mammalian heterochromatin proteins (HP1alpha, HP1beta, HP1gamma). However, the 
      nontranscribed sequences were found predominantly at the nuclear periphery or at 
      the nucleoli, whereas genes tended to localize on chromosome surfaces exposed to 
      the nuclear interior.
FAU - Scheuermann, Markus O
AU  - Scheuermann MO
AD  - Division of Molecular Genetics, Deutsches Krebsforschungszentrum, D-69120 
      Heidelberg, Germany.
FAU - Tajbakhsh, Jian
AU  - Tajbakhsh J
FAU - Kurz, Anette
AU  - Kurz A
FAU - Saracoglu, Kaan
AU  - Saracoglu K
FAU - Eils, Roland
AU  - Eils R
FAU - Lichter, Peter
AU  - Lichter P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
SB  - IM
EIN - Exp Cell Res. 2005 Mar 10;304(1):337-8
MH  - Base Sequence
MH  - Cell Nucleus/*genetics/*ultrastructure
MH  - Chromobox Protein Homolog 5
MH  - Chromosomes
MH  - Female
MH  - Fibroblasts
MH  - *Gene Components
MH  - Gene Expression Profiling
MH  - *Genes
MH  - HeLa Cells
MH  - Humans
MH  - Imaging, Three-Dimensional
MH  - Interphase/*genetics
MH  - Male
EDAT- 2004/11/09 09:00
MHDA- 2005/01/04 09:00
CRDT- 2004/11/09 09:00
PHST- 2004/03/31 00:00 [received]
PHST- 2004/07/03 00:00 [revised]
PHST- 2004/11/09 09:00 [pubmed]
PHST- 2005/01/04 09:00 [medline]
PHST- 2004/11/09 09:00 [entrez]
AID - S0014-4827(04)00447-1 [pii]
AID - 10.1016/j.yexcr.2004.08.031 [doi]
PST - ppublish
SO  - Exp Cell Res. 2004 Dec 10;301(2):266-79. doi: 10.1016/j.yexcr.2004.08.031.