PMID- 15531429
OWN - NLM
STAT- MEDLINE
DCOM- 20050207
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Linking)
VI  - 112
IP  - 15
DP  - 2004 Nov
TI  - Androgenic and estrogenic response of green mussel extracts from Singapore's 
      coastal environment using a human cell-based bioassay.
PG  - 1467-71
AB  - In the last decade, evidence of endocrine disruption in biota exposed to 
      environmental pollutants has raised serious concern. Human cell-based bioassays 
      have been developed to evaluate induced androgenic and estrogenic activities of 
      chemical compounds. However, bioassays have been sparsely applied to 
      environmental samples. In this study we present data on sex hormone activities in 
      the green mussel, Perna viridis, in Singapore's coastal waters. P.viridis is a 
      common bioindicator of marine contamination, and this study is a follow-up to an 
      earlier investigation that reported the presence of sex hormone activities in 
      seawater samples from Singapore's coastal environment. Specimens were collected 
      from eight locations around the Singapore coastline and analyzed for persistent 
      organic pollutants (POPs) and heavy metals. Tissue extracts were then screened 
      for activities on androgen receptors (ARs) and estrogen receptors (ER-alpha and 
      ER-beta) using a reporter gene bioassay based on a HeLa human cell line. Mussel 
      extracts alone did not exhibit AR activity, but in the presence of the reference 
      androgenic hormone dihydrotestosterone (DHT), activities were up to 340% higher 
      than those observed for DHT alone. Peak activities were observed in locations 
      adjacent to industrial and shipping activities. Estrogenic activities of the 
      mussel extract both alone and in the presence of reference hormone were positive. 
      Correlations were statistically investigated between sex hormone activities, 
      levels of pollutants in the mussel tissues, and various biological parameters 
      (specimen size, sex ratio, lipid and moisture content). Significant correlations 
      exist between AR activities, in the presence of DHT, and total concentration of 
      POPs (r= 0.725, p < 0.05).
FAU - Bayen, Stephane
AU  - Bayen S
AD  - Department of Chemistry and the Tropical Marine Science Institute, National 
      University of Singapore, Republic of Singapore. scip0153@nus.eud.sg
FAU - Gong, Yinhan
AU  - Gong Y
FAU - Chin, Hong Soon
AU  - Chin HS
FAU - Lee, Hian Kee
AU  - Lee HK
FAU - Leong, Yong Eu
AU  - Leong YE
FAU - Obbard, Jeffrey Philip
AU  - Obbard JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Organic Chemicals)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Bivalvia/*chemistry
MH  - Endocrine System/drug effects
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Organic Chemicals
MH  - Receptors, Androgen/*drug effects
MH  - Receptors, Estrogen/*drug effects
MH  - Seawater/chemistry
MH  - Singapore
MH  - Water Pollutants, Chemical/*toxicity
PMC - PMC1247608
EDAT- 2004/11/09 09:00
MHDA- 2005/02/08 09:00
PMCR- 2004/11/01
CRDT- 2004/11/09 09:00
PHST- 2004/11/09 09:00 [pubmed]
PHST- 2005/02/08 09:00 [medline]
PHST- 2004/11/09 09:00 [entrez]
PHST- 2004/11/01 00:00 [pmc-release]
AID - ehp0112-001467 [pii]
AID - 10.1289/ehp.6990 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2004 Nov;112(15):1467-71. doi: 10.1289/ehp.6990.