PMID- 15533683
OWN - NLM
STAT- MEDLINE
DCOM- 20050412
LR  - 20131121
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 36
IP  - 4
DP  - 2004 Nov 19
TI  - Simple and rapid determination of adenosine in human synovial fluid with high 
      performance liquid chromatography-mass spectrometry.
PG  - 877-82
AB  - A simple, fast, sensitive and selective reversed-phase high performance liquid 
      chromatography-mass spectrometry coupling with an electrospray ionization (ESI) 
      interface method is described for the determination of adenosine in human 
      synovial fluid. This method involved the use of the [M + H](+)ions of adenosine 
      and 2-chloroadenosine (internal standard for the assay) at m/z 268 and 302 in 
      positive ion mode with selective ion monitoring (SIM). Separation was carried out 
      on a 2.0 x 150 mm Shimadzu VP-ODS column by using an isocratic elution with a 
      mobile phase consisting of water (94%),methanol (5%) and formic acid (1%). No 
      interference with the components of the biological matrix was observed in the 
      determination conditions. The calibration curve was linear in the range of 
      0.2-140 microgml(-1). The limits of quantification (LOQ) and detection (LOD) were 
      0.2 and 0.03 microgml(-1), respectively. The standard recoveries were between 
      93.3 and 104.0%. The method was successfully applied to determination of 
      adenosine in some synovial fluids of patients affected by rheumatoid arthritis.
FAU - Huang, Lan-Fang
AU  - Huang LF
AD  - College of Chemistry and Chemical Engineering of Central South University, 
      Changsha 410083, P. R. China. fl18huang@yahoo.com.cn
FAU - Guo, Fang-Qiu
AU  - Guo FQ
FAU - Liang, Yi-Zeng
AU  - Liang YZ
FAU - Li, Bo-Yang
AU  - Li BY
FAU - Cheng, Ben-Mei
AU  - Cheng BM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*analysis
MH  - Chromatography, High Pressure Liquid/methods
MH  - Humans
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Synovial Fluid/*chemistry
EDAT- 2004/11/10 09:00
MHDA- 2005/04/13 09:00
CRDT- 2004/11/10 09:00
PHST- 2004/05/19 00:00 [received]
PHST- 2004/07/27 00:00 [revised]
PHST- 2004/07/31 00:00 [accepted]
PHST- 2004/11/10 09:00 [pubmed]
PHST- 2005/04/13 09:00 [medline]
PHST- 2004/11/10 09:00 [entrez]
AID - S0731-7085(04)00357-7 [pii]
AID - 10.1016/j.jpba.2004.07.038 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2004 Nov 19;36(4):877-82. doi: 10.1016/j.jpba.2004.07.038.