PMID- 15537073
OWN - NLM
STAT- MEDLINE
DCOM- 20050329
LR  - 20191210
IS  - 1615-9306 (Print)
IS  - 1615-9306 (Linking)
VI  - 27
IP  - 14
DP  - 2004 Oct
TI  - Coupled-column liquid chromatography method with photochemically induced 
      derivatization for the direct determination of benzoylureas in vegetables.
PG  - 1173-80
AB  - The coupled-column (LC-LC) system, consisting of a first column packed with 
      internal surface reversed phase (ISRP) (50 x 4.6mm ID) and a Chrompack C18 (100 x 
      4.6 mm ID) as second column, allowed the simultaneous determination of five 
      benzoylurea insecticides in dichloromethane (CH2Cl2) extracts of vegetable 
      samples without any clean-up step. This system was combined with a 
      photochemically induced fluorescence (PIF) post-column derivatization in order to 
      provide strongly fluorescent photoproducts from the non-fluorescent benzoylureas. 
      Limits of detection ranged from 0.21 to 0.98 microg L(-1) of pesticide 
      (equivalent to 0.14-0.65 microg kg(-1) in vegetable samples) and limits of 
      determination ranged from 4.0 to 10.0 microg L(-1) (equivalent to 2.7-6.7 microg 
      kg(-1)). Linearity of the method was established between 2 and 1800 microg L(-1), 
      depending upon the compound. Validation of the total method was performed by 
      randomly analyzing recoveries of four vegetable samples (aubergine, cucumber, 
      green bean, and tomato) spiked at two levels of concentration (10.0 and 33.3 
      microg kg(-1)). The combination of the LC-LC system with PIF detection provides a 
      sensitive, selective, and rapid method for the determination of pesticides in 
      vegetable samples at levels lower than the maximum residue levels (MRLs) 
      established for these compounds by Spanish legislation.
FAU - Garcia, Maria Dolores Gil
AU  - Garcia MD
AD  - Department of Analytical Chemistry, University of Almeria, La Canada de San 
      Urbano, 04120 Almeria, Spain. mdgil@ual.es
FAU - Martinez, Dolores Barranco
AU  - Martinez DB
FAU - Galera, Maria Martinez
AU  - Galera MM
FAU - Vazquez, Piedad Parrilla
AU  - Vazquez PP
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Benzamides)
RN  - 0 (Insecticides)
RN  - 0 (Pesticide Residues)
RN  - 0 (Phenylurea Compounds)
SB  - IM
MH  - Animals
MH  - Benzamides/*analysis
MH  - Calibration
MH  - Chromatography, Liquid/instrumentation/*methods
MH  - Insecticides/*chemistry
MH  - Pesticide Residues/analysis
MH  - Phenylurea Compounds/*analysis
MH  - Photochemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Vegetables/*chemistry
EDAT- 2004/11/13 09:00
MHDA- 2005/03/30 09:00
CRDT- 2004/11/13 09:00
PHST- 2004/11/13 09:00 [pubmed]
PHST- 2005/03/30 09:00 [medline]
PHST- 2004/11/13 09:00 [entrez]
AID - 10.1002/jssc.200301661 [doi]
PST - ppublish
SO  - J Sep Sci. 2004 Oct;27(14):1173-80. doi: 10.1002/jssc.200301661.