PMID- 15538109
OWN - NLM
STAT- MEDLINE
DCOM- 20050315
LR  - 20191109
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 13
IP  - 4
DP  - 2004 Dec
TI  - Paraffin section interphase fluorescence in situ hybridization in the diagnosis 
      and classification of non-hodgkin lymphomas.
PG  - 197-206
AB  - Cytogenetic data can contribute valuable information that may assist in the 
      diagnosis and classification of non-Hodgkin lymphomas and may in some cases also 
      provide prognostic information. Interphase fluorescence in situ hybridization 
      (FISH) studies offer the ability to assess for characteristic cytogenetic 
      abnormalities even when material for standard metaphase cytogenetic analysis is 
      not available. This review discusses the use of FISH in paraffin-embedded 
      material with particular attention paid to the use of intact thin paraffin 
      sections. The basic principles of FISH analysis are summarized, the advantages 
      and disadvantages of analysis of thin paraffin sections rather than intact nuclei 
      are discussed, and the more commonly encountered artifacts are considered. Each 
      of the well-characterized cytogenetic abnormalities that are associated with 
      particular types of non-Hodgkin lymphoma and can be detected with commercially 
      available FISH probes is discussed individually. In particular, their incidence 
      in various types of lymphoma is reviewed, the types of commercially available 
      FISH probes to detect such abnormalities are discussed, and clinical situations 
      where such analysis can be of diagnostic utility are described.
FAU - Cook, James Robert
AU  - Cook JR
AD  - Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, OH 
      44195, USA. Cookj2@ccf.org
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/*genetics
MH  - Lymphoma, Non-Hodgkin/classification/*diagnosis/genetics
MH  - Paraffin Embedding
RF  - 66
EDAT- 2004/11/13 09:00
MHDA- 2005/03/16 09:00
CRDT- 2004/11/13 09:00
PHST- 2004/11/13 09:00 [pubmed]
PHST- 2005/03/16 09:00 [medline]
PHST- 2004/11/13 09:00 [entrez]
AID - 00019606-200412000-00001 [pii]
AID - 10.1097/01.pdm.0000135286.05198.89 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2004 Dec;13(4):197-206. doi: 
      10.1097/01.pdm.0000135286.05198.89.