PMID- 15538959
OWN - NLM
STAT- MEDLINE
DCOM- 20050217
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 28
IP  - 8
DP  - 2004 Nov-Dec
TI  - Effectiveness of multiple internal standards: deuterated analogues of 
      methylenedioxymethamphetamine, methylenedioxyamphetamine, methamphetamine, and 
      amphetamine.
PG  - 650-4
AB  - With the increasing abuse of methylenedioxymethamphetamine (MDMA) thereby 
      requiring analysis, we have undertaken a systematic evaluation on parameters 
      associated with the analysis of MDMA and related compounds, including 
      methylenedioxyamphetamine (MDA), methamphetamine (MA), and amphetamine (AM). 
      Parameters studied included three solid-phase adsorbents, five derivatization 
      reagents, and four deuterated internal standards (IS). This report examines 
      whether differences in quantitation data derived from the use of four ISs (one 
      for each analyte) and two ISs (one for AM and MA, one for MDA and MDMA) are 
      statistically significant. Two types of samples were included in this study. The 
      first type (Type I) included four replicate sets of standard solutions prepared 
      in urine matrix. All analytes (AM, MA, MDA, and MDMA) were included in all 
      samples, and these analytes' concentrations in each set were at five levels (100, 
      250, 500, 1000, and 2000 ng/mL). Four deuterated analogues (MA-d(8), AM-d(8), 
      MDMA-d(5), and MDA-d(5)) at 500 ng/mL were also included in all solutions. The 
      second type of samples (Type II) included 25 case urine specimens. Most of these 
      specimens contained MA/AM and/or MDMA/MDA. The specific objective of this study 
      is to determine whether the 4-IS approach can indeed generate better quantitative 
      data than a less-costly 2-IS. For Type I samples, where the true concentrations 
      of the analytes are known, two-sample t-test is adapted to examine whether the 
      two sets of prediction errors (i.e., known concentration minus calculated 
      concentration) resulting from the 4-IS and the 2-IS approaches are statistically 
      different. For Type II samples, where the analytes' true concentrations are 
      unknown, one-sample t-test was adapted to determine whether the difference of the 
      quantitation results derived from the 4-IS and the 2-IS approaches is 
      statistically significant. Statistical analysis of quantitation data derived from 
      Types I and II samples indicates that differences in MDA and MDMA concentrations 
      resulting from the use of one (MDA-d(5) or MDMA-d(5)) or two (MDA-d(5) and 
      MDMA-d(5)) are statistically nonsignificant. On the other hand, similar analysis 
      on data derived from Type I samples indicate the use of the analytes' respective 
      deuterated analogues as the ISs appear to generate better quantitative data for 
      AM and MA.
FAU - Lin, Dong-Liang
AU  - Lin DL
AD  - Institute of Forensic Medicine, Ministry of Justice, Taipei, Taiwan.
FAU - Liu, Hsiu-Chuan
AU  - Liu HC
FAU - Yin, Rea-Ming
AU  - Yin RM
FAU - Chen, Dung-Tsa
AU  - Chen DT
FAU - Soong, Seng-Jaw
AU  - Soong SJ
FAU - Liu, Ray H
AU  - Liu RH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - AR09D82C7G (Deuterium)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*urine
MH  - Deuterium
MH  - Humans
MH  - Methamphetamine/*urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*urine
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Substance Abuse Detection/*methods/standards
EDAT- 2004/11/13 09:00
MHDA- 2005/02/18 09:00
CRDT- 2004/11/13 09:00
PHST- 2004/11/13 09:00 [pubmed]
PHST- 2005/02/18 09:00 [medline]
PHST- 2004/11/13 09:00 [entrez]
AID - 10.1093/jat/28.8.650 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2004 Nov-Dec;28(8):650-4. doi: 10.1093/jat/28.8.650.