PMID- 15539508
OWN - NLM
STAT- MEDLINE
DCOM- 20050511
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 11
IP  - 6
DP  - 2004 Nov
TI  - New latex bead agglutination assay for differential diagnosis of cattle infected 
      with Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis.
PG  - 1070-4
AB  - Extensive studies have shown that the current assays used to identify cattle 
      infected with Mycobacterium bovis or Mycobacterium avium subsp. paratuberculosis 
      are not sufficiently sensitive and specific to detect all infected animals, 
      especially animals recently infected with the pathogens. In the present report we 
      show that these limitations might be overcome with a latex bead agglutination 
      assay (LBAA). With the specific immunodominant epitope (ESAT6-p) of M. bovis, we 
      developed an LBAA and enzyme immunoassay (EIA) for that purpose and compared them 
      with the "gold standard" culture method and skin test for their efficacy in 
      detecting bovine tuberculosis. When sera from control healthy cows (n = 10), M. 
      avium subsp. paratuberculosis-positive cattle (naturally infected, n = 16; 
      experimentally infected, n = 8), and M. bovis-positive cattle (naturally 
      infected, n = 49;experimentally infected, n = 20) were applied to an EIA and an 
      LBAA developed with ESAT6-p, the two tests showed similar sensitivity (97.1% by 
      EIA, 95.7% by LBAA), high specificity (94.2% by EIA, 100% by LBAA), and a 
      positive correlation (kappa value, 0.85; correlation rate, 93.2%; correlation 
      coefficient, 0.64). Receiver operating characteristic analysis of EIA results and 
      comparison with the culture method determined a suitable cutoff value at 0.469, 
      with an area under the curve of 0.991 (95% confidence interval, 0.977 to 1.0). As 
      LBAA didn't show any positive reactions with sera from uninfected control cows or 
      M. avium subsp. paratuberculosis-infected cattle, which were confirmed to be free 
      of M. bovis by culture or PCR, LBAA using the ESAT6-p can be a rapid and useful 
      M. bovis diagnostic assay. The data suggest that rapid, sensitive, and specific 
      assays can be developed with peptides containing immunodominant epitopes present 
      in proteins uniquely expressed in M. bovis or M. avium subsp. paratuberculosis 
      for differential diagnosis of cattle infected with M. bovis or M. avium subsp. 
      paratuberculosis.
FAU - Koo, Hye Cheong
AU  - Koo HC
AD  - Department of Microbiology, College of Veterinary Medicine, and School of 
      Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
FAU - Park, Yong Ho
AU  - Park YH
FAU - Ahn, Jongsam
AU  - Ahn J
FAU - Waters, W Ray
AU  - Waters WR
FAU - Hamilton, Mary Jo
AU  - Hamilton MJ
FAU - Barrington, George
AU  - Barrington G
FAU - Mosaad, Abdelaziz A
AU  - Mosaad AA
FAU - Palmer, Mitch V
AU  - Palmer MV
FAU - Shin, Sang
AU  - Shin S
FAU - Davis, William C
AU  - Davis WC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunodominant Epitopes)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/*analysis
MH  - Cattle
MH  - Diagnosis, Differential
MH  - Immunodominant Epitopes/immunology
MH  - Latex Fixation Tests/methods
MH  - Mycobacterium avium subsp. paratuberculosis/*immunology
MH  - Mycobacterium bovis/*immunology
MH  - Paratuberculosis/*diagnosis
MH  - Predictive Value of Tests
MH  - Tuberculosis, Bovine/*diagnosis
PMC - PMC524750
EDAT- 2004/11/13 09:00
MHDA- 2005/05/12 09:00
PMCR- 2004/11/01
CRDT- 2004/11/13 09:00
PHST- 2004/11/13 09:00 [pubmed]
PHST- 2005/05/12 09:00 [medline]
PHST- 2004/11/13 09:00 [entrez]
PHST- 2004/11/01 00:00 [pmc-release]
AID - 11/6/1070 [pii]
AID - 0072-04 [pii]
AID - 10.1128/CDLI.11.6.1070-1074.2004 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2004 Nov;11(6):1070-4. doi: 
      10.1128/CDLI.11.6.1070-1074.2004.