PMID- 15540905
OWN - NLM
STAT- MEDLINE
DCOM- 20050216
LR  - 20191026
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 80
IP  - 3
DP  - 2004 Oct
TI  - In vitro generation of functional dendritic cells from human umbilical cord blood 
      CD34+ cells by a 2-step culture method.
PG  - 281-6
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells in terms of 
      initiating primary T-cell-dependent immune responses. We devised a 2-step culture 
      method for obtaining sufficient numbers of functional DCs from umbilical cord 
      blood (CB) CD34+ cells. In the first step, CB CD34+ cells were expanded by 
      stimulation with early-acting cytokines such as stem cell factor (SCF), flt3 
      ligand (FL), and thrombopoietin (TPO) to amplify the hematopoietic progenitor 
      cells. In the second step, granulocyte-macrophage colony-stimulating factor and 
      interleukin 4 were added, and incubation was continued for another 5 days to 
      induce differentiation of the expanded cells into DCs. During the first step of 
      culturing with TPO, SCF, and FL, the total numbers of nucleated cells gradually 
      increased, peaking at 4 weeks (245.3-fold). During the second step, expression of 
      CD1a, CD83, and CD86 increased. Electron microscopic findings showed that these 
      cells had cytosolic expansion to form dendrites and major histocompatibility 
      complex class II compartments, which are characteristic of DCs. Functional 
      analyses revealed that these cells had phagocytic activity and were capable of 
      stimulating allogeneic T-cells in vitro.
FAU - Ryu, Kyung Ha
AU  - Ryu KH
AD  - Department of Pediatrics, Ewha Womans University College of Medicine, Ewha 
      Medical Research Center, Seoul, Korea.
FAU - Cho, Su Jin
AU  - Cho SJ
FAU - Jung, Yoon Jae
AU  - Jung YJ
FAU - Seoh, Ju Young
AU  - Seoh JY
FAU - Kie, Jeong Hae
AU  - Kie JH
FAU - Koh, Sang Hyeok
AU  - Koh SH
FAU - Kang, Hyoung Jin
AU  - Kang HJ
FAU - Ahn, Hyo Seop
AU  - Ahn HS
FAU - Shin, Hee Young
AU  - Shin HY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Antigens, CD34)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Antigens, CD34
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cytokines/pharmacology
MH  - Dendrites
MH  - Dendritic Cells/*cytology
MH  - Fetal Blood/cytology
MH  - Histocompatibility Antigens Class II
MH  - Humans
MH  - Microscopy, Electron
EDAT- 2004/11/16 09:00
MHDA- 2005/02/17 09:00
CRDT- 2004/11/16 09:00
PHST- 2004/11/16 09:00 [pubmed]
PHST- 2005/02/17 09:00 [medline]
PHST- 2004/11/16 09:00 [entrez]
AID - 10.1532/ijh97.a10406 [doi]
PST - ppublish
SO  - Int J Hematol. 2004 Oct;80(3):281-6. doi: 10.1532/ijh97.a10406.