PMID- 15541290
OWN - NLM
STAT- MEDLINE
DCOM- 20041214
LR  - 20161124
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 293
IP  - 1-2
DP  - 2004 Oct
TI  - Isolating pure populations of monocytes from the blood of pregnant women: 
      comparison of flotation in iodixanol with elutriation.
PG  - 215-8
AB  - Observations that the innate arm of the immune system is upregulated in pregnancy 
      have highlighted the need for methods of isolating pure populations of monocytes 
      for studies into pregnancy and pre-eclampsia without activating them during the 
      isolation process. Density gradient centrifugation using iodixanol is a useful 
      method for isolating relatively pure populations of unactivated monocytes from 
      human blood but has not been validated in pregnant subjects. We compared the 
      ability of monocytes isolated from pregnant women by density gradient 
      centrifugation using iodixanol (n=6) with monocytes isolated by countercurrent 
      centrifugal elutriation (n=6) in terms of their ability to produce interleukin-6 
      (IL-6) and monocyte chemoattractant protein-1 (MCP-1) under basal conditions and 
      after stimulation with bacterial lipopolysaccharide (LPS). Under basal 
      conditions, monocytes isolated by density gradient centrifugation produced low 
      amounts of IL-6 and MCP-1. Production of IL-6 and MCP-1 after stimulation of the 
      monocytes with LPS was much greater (p<0.01). There was no statistically 
      significant difference between the two methods in terms of stimulated levels of 
      either cytokine.
FAU - Nutt, Jeffrey C
AU  - Nutt JC
AD  - Perinatal and Renal Research Groups, Kolling Institute of Medical Research, Royal 
      North Shore Hospital, St Leonards, NSW, Australia. jnutt@med.usyd.edu.au
FAU - Willis, Catherine C
AU  - Willis CC
FAU - Morris, Jonathan M
AU  - Morris JM
FAU - Gallery, Eileen D M
AU  - Gallery ED
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Contrast Media)
RN  - 0 (Triiodobenzoic Acids)
RN  - HW8W27HTXX (iodixanol)
SB  - IM
MH  - Antigens, CD/immunology
MH  - Antigens, Differentiation, Myelomonocytic/immunology
MH  - Cell Separation/*methods
MH  - Contrast Media/*pharmacology
MH  - Female
MH  - Humans
MH  - *Monocytes/drug effects/immunology
MH  - Pregnancy
MH  - Triiodobenzoic Acids/*pharmacology
EDAT- 2004/11/16 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/11/16 09:00
PHST- 2003/04/28 00:00 [received]
PHST- 2004/05/11 00:00 [revised]
PHST- 2004/05/28 00:00 [accepted]
PHST- 2004/11/16 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/11/16 09:00 [entrez]
AID - S0022-1759(04)00278-9 [pii]
AID - 10.1016/j.jim.2004.05.011 [doi]
PST - ppublish
SO  - J Immunol Methods. 2004 Oct;293(1-2):215-8. doi: 10.1016/j.jim.2004.05.011.