PMID- 15541771
OWN - NLM
STAT- MEDLINE
DCOM- 20050520
LR  - 20171116
IS  - 0047-6374 (Print)
IS  - 0047-6374 (Linking)
VI  - 125
IP  - 10-11
DP  - 2004 Oct-Nov
TI  - Novel regulatory factors of HSF-1 activation: facts and perspectives regarding 
      their involvement in the age-associated attenuation of the heat shock response.
PG  - 767-75
AB  - An attenuated response to stress is characteristic of senescence. Heat shock 
      (HS), a significant form of stress, is delayed and reduced in aging organisms. In 
      the response to heat shock, heat shock factor 1 (HSF-1) is activated by 
      trimerization of its monomeric subunits. This then initiates the transcription of 
      a series of heat shock genes (hsp genes) that encode chaperone proteins 
      protective against heat stress. Using a promoter binding electromobility shift 
      assay (EMSA), we have found no activation of this transcription factor in the 
      brains of old (36 months) rats in response to exposure to 41 degrees C for 1h 
      while strong activation is elicited in young (6 months) animals. Since brains of 
      young and old rats had approximately the same amount of HSF-1 subunits, we 
      anticipated the presence of auxiliary regulatory factors essential for the 
      activation of HSF-1 and the initiation of heat shock gene transcription. We 
      describe three novel auxiliary factors--the proteins I-HSF [HSF inhibitor] and 
      elongation factor-1 alpha (EF-1alpha) and a large non-coding RNA (HSR)--that 
      participate in regulation and activation of HSF-1 in early stages of heat shock 
      gene transcription. I-HSF inhibits trimerization of HSF-1 at normal temperatures. 
      HSR and EF-1alpha form a complex with HSF-1 and facilitate its trimerization and 
      binding to heat shock element (HSE) in the promoters of hsps. It is proposed that 
      structural changes in any one or a combination of these factors in response to 
      heat shock may contribute to the age-associated attenuation in the response to 
      stress.
FAU - Shamovsky, Ilya
AU  - Shamovsky I
AD  - Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, 
      Israel.
FAU - Gershon, David
AU  - Gershon D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mech Ageing Dev
JT  - Mechanisms of ageing and development
JID - 0347227
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Peptide Elongation Factor 1)
RN  - 0 (RNA, Nuclear)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Brain/metabolism
MH  - DNA-Binding Proteins/antagonists & inhibitors/*metabolism
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Heat Shock Transcription Factors
MH  - Heat Stress Disorders/*metabolism
MH  - Heat-Shock Proteins/biosynthesis
MH  - Peptide Elongation Factor 1/*metabolism
MH  - RNA, Nuclear/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription Factors
EDAT- 2004/11/16 09:00
MHDA- 2005/05/21 09:00
CRDT- 2004/11/16 09:00
PHST- 2004/11/16 09:00 [pubmed]
PHST- 2005/05/21 09:00 [medline]
PHST- 2004/11/16 09:00 [entrez]
AID - S0047-6374(04)00167-8 [pii]
AID - 10.1016/j.mad.2004.07.006 [doi]
PST - ppublish
SO  - Mech Ageing Dev. 2004 Oct-Nov;125(10-11):767-75. doi: 10.1016/j.mad.2004.07.006.