PMID- 15542841
OWN - NLM
STAT- MEDLINE
DCOM- 20050104
LR  - 20181113
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 24
IP  - 23
DP  - 2004 Dec
TI  - Proapoptotic function of the MET tyrosine kinase receptor through caspase 
      cleavage.
PG  - 10328-39
AB  - The MET tyrosine kinase, the receptor of hepatocyte growth factor-scatter factor 
      (HGF/SF), is known to be essential for normal development and cell survival. We 
      report that stress stimuli induce the caspase-mediated cleavage of MET in 
      physiological cellular targets, such as epithelial cells, embryonic hepatocytes, 
      and cortical neurons. Cleavage occurs at aspartic residue 1000 within the SVD 
      site of the juxtamembrane region, independently of the crucial docking tyrosine 
      residues Y1001 or Y1347 and Y1354. This cleavage generates an intracellular 
      40-kDa MET fragment containing the kinase domain. The p40 MET fragment itself 
      causes apoptosis of MDCK epithelial cells and embryonic cortical neurons, whereas 
      its kinase-dead version is impaired in proapoptotic activity. Finally, HGF/SF 
      treatment does not favor MET cleavage and apoptosis, confirming the known 
      survival role of ligand-activated MET. Our results show that stress stimuli 
      convert the MET survival receptor into a proapoptotic factor.
FAU - Tulasne, David
AU  - Tulasne D
AD  - CNRS UMR 8117, Institut de Biologie de Lille, Institut Pasteur de Lille, B.P. 
      447, 59021 Lille, France. david.tulasne@ibl.fr
FAU - Deheuninck, Julien
AU  - Deheuninck J
FAU - Lourenco, Filipe Calheiros
AU  - Lourenco FC
FAU - Lamballe, Fabienne
AU  - Lamballe F
FAU - Ji, Zongling
AU  - Ji Z
FAU - Leroy, Catherine
AU  - Leroy C
FAU - Puchois, Emilie
AU  - Puchois E
FAU - Moumen, Anice
AU  - Moumen A
FAU - Maina, Flavio
AU  - Maina F
FAU - Mehlen, Patrick
AU  - Mehlen P
FAU - Fafeur, Veronique
AU  - Fafeur V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cytokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Ligands)
RN  - 0 (Recombinant Proteins)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Apoptosis
MH  - Aspartic Acid/chemistry/metabolism
MH  - Blotting, Western
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dogs
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Hepatocytes/metabolism
MH  - In Situ Nick-End Labeling
MH  - Ligands
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Neurons/metabolism
MH  - Plasmids/metabolism
MH  - Protein Biosynthesis
MH  - Protein Structure, Tertiary
MH  - Proto-Oncogene Proteins c-met/metabolism/*physiology
MH  - Recombinant Proteins/chemistry
MH  - Transfection
MH  - Tyrosine/chemistry
PMC - PMC529022
EDAT- 2004/11/16 09:00
MHDA- 2005/01/05 09:00
PMCR- 2004/12/01
CRDT- 2004/11/16 09:00
PHST- 2004/11/16 09:00 [pubmed]
PHST- 2005/01/05 09:00 [medline]
PHST- 2004/11/16 09:00 [entrez]
PHST- 2004/12/01 00:00 [pmc-release]
AID - 24/23/10328 [pii]
AID - 0531-04 [pii]
AID - 10.1128/MCB.24.23.10328-10339.2004 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2004 Dec;24(23):10328-39. doi: 10.1128/MCB.24.23.10328-10339.2004.