PMID- 15543578
OWN - NLM
STAT- MEDLINE
DCOM- 20050809
LR  - 20081121
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 94
IP  - 3
DP  - 2005 Feb 15
TI  - Histone acetyltransferase activity of p300 enhances the activation of IL-18 
      promoter.
PG  - 566-72
AB  - Interleukin-18 (IL-18), an important regulator of innate and acquired immune 
      responses expressed from a variety of cell types, is a pleiotropic cytokine in 
      the development of T helper type 1 (Thl) cells. The p300/CBP (CREB-binding 
      protein) coactivator proteins are important histone acetyltransferases (HATs) 
      that regulate the transcription of many genes. Whether p300/CBP play a role in 
      the IL-18 expression has not been investigated previously. In this study, we 
      analyzed the roles of p300 in the regulation of mouse IL-18 by using RT-PCR and a 
      series of co-transfection studies. We showed that p300 had a stimulating effect 
      on the endogenous IL-18 mRNA synthesis and on the activity of IL-18 p1 promoter. 
      The results also showed that IL-18 p1 promoter activity was enhanced by p300 in a 
      dose-dependent manner. Moreover, the p300-mediated activation function can be 
      suppressed by the adenovirus E1A protein, which inhibits the HAT function of 
      p300. Also, a mutation in p300 HAT region abolished the effect of p300 on IL-18 
      activation. These data further indicate that the acetylase activity of p300 was 
      indispensable to its function. Furthermore, we found that p300 was able to 
      enhance the effect of the transcription factor c-Fos on activation of the IL-18 
      promoter. Data presented in this paper implicate important roles of p300 in the 
      transcriptional regulation of IL-18.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Sun, Haijing
AU  - Sun H
AD  - Institute of Genetics and Cytology, Northeast Normal University, Changchun 
      130024, P.R. China.
FAU - Lu, Jun
AU  - Lu J
FAU - Xu, Xin
AU  - Xu X
FAU - Jin, Shunzi
AU  - Jin S
FAU - Wang, Xiuli
AU  - Wang X
FAU - Wei, Liang
AU  - Wei L
FAU - Dong, Mei
AU  - Dong M
FAU - Huang, Baiqu
AU  - Huang B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (p300-CBP Transcription Factors)
RN  - EC 2.3.1.48 (p300-CBP-associated factor)
SB  - IM
MH  - Acetyltransferases/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - Cell Cycle Proteins/*metabolism
MH  - DNA Primers
MH  - Histone Acetyltransferases
MH  - Interleukin-18/*genetics
MH  - Mice
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - p300-CBP Transcription Factors
EDAT- 2004/11/16 09:00
MHDA- 2005/08/10 09:00
CRDT- 2004/11/16 09:00
PHST- 2004/11/16 09:00 [pubmed]
PHST- 2005/08/10 09:00 [medline]
PHST- 2004/11/16 09:00 [entrez]
AID - 10.1002/jcb.20194 [doi]
PST - ppublish
SO  - J Cell Biochem. 2005 Feb 15;94(3):566-72. doi: 10.1002/jcb.20194.