PMID- 15545660
OWN - NLM
STAT- MEDLINE
DCOM- 20050715
LR  - 20240109
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 169
IP  - 2
DP  - 2005 Feb
TI  - Selection on Glycine beta-1,3-endoglucanase genes differentially inhibited by a 
      Phytophthora glucanase inhibitor protein.
PG  - 1009-19
AB  - Plant endo-beta-1,3-glucanases (EGases) degrade the cell wall polysaccharides of 
      attacking pathogens and release elicitors of additional plant defenses. Isozymes 
      EGaseA and EGaseB of soybean differ in susceptibility to a glucanase inhibitor 
      protein (GIP1) produced by Phytophthora sojae, a major soybean pathogen. EGaseA, 
      the major elicitor-releasing isozyme, is a high-affinity ligand for GIP1, which 
      completely inhibits it, whereas EGaseB is unaffected by GIP1. We tested for 
      departures from neutral evolution on the basis of partial sequences of EGaseA and 
      EGaseB from 20 widespread accessions of Glycine soja (the wild progenitor of 
      soybean), from 4 other Glycine species, and across dicotyledonous plants. G. soja 
      exhibited little intraspecific variation at either locus. Phylogeny-based codon 
      evolution models detected strong evidence of positive selection on Glycine EGaseA 
      and weaker evidence for selection on dicot EGases and Glycine EGaseB. Positively 
      selected peptide sites were identified and located on a structural model of EGase 
      bound to GIP1. Positively selected sites and highly variable sites were found 
      disproportionately within 4.5 angstroms of bound GIP1. Low variation within G. 
      soja EGases, coupled with positive selection in both Glycine and dicot lineages 
      and the proximity of rapidly evolving sites to GIP1, suggests an arms race 
      involving repeated adaptation to pathogen attack and inhibition.
FAU - Bishop, J G
AU  - Bishop JG
AD  - School of Biological Sciences, Washington State University, Vancouver, Washington 
      98686-9600, USA. bishop@vancouver.wsu.edu
FAU - Ripoll, D R
AU  - Ripoll DR
FAU - Bashir, S
AU  - Bashir S
FAU - Damasceno, C M B
AU  - Damasceno CM
FAU - Seeds, J D
AU  - Seeds JD
FAU - Rose, J K C
AU  - Rose JK
LA  - eng
SI  - GENBANK/AY461847
SI  - GENBANK/AY466133
SI  - GENBANK/AY466134
SI  - GENBANK/AY466135
SI  - GENBANK/AY466136
SI  - GENBANK/AY466137
SI  - GENBANK/AY466138
SI  - GENBANK/AY466139
SI  - GENBANK/AY466140
SI  - GENBANK/AY466141
SI  - GENBANK/AY466142
SI  - GENBANK/AY466143
SI  - GENBANK/AY466144
SI  - GENBANK/AY466145
SI  - GENBANK/AY466146
SI  - GENBANK/AY466147
SI  - GENBANK/AY466148
SI  - GENBANK/AY466149
SI  - GENBANK/AY466150
SI  - GENBANK/AY466151
SI  - GENBANK/AY466152
SI  - GENBANK/AY466153
SI  - GENBANK/AY466154
SI  - GENBANK/AY466155
SI  - GENBANK/AY466156
SI  - GENBANK/AY468381
SI  - GENBANK/AY468382
SI  - GENBANK/AY468383
SI  - GENBANK/AY468384
SI  - GENBANK/AY468385
SI  - GENBANK/AY468386
SI  - GENBANK/AY468387
SI  - GENBANK/AY468388
SI  - GENBANK/AY468389
SI  - GENBANK/AY468390
SI  - GENBANK/AY468391
SI  - GENBANK/AY468392
SI  - GENBANK/AY468393
SI  - GENBANK/AY468394
SI  - GENBANK/AY468395
SI  - GENBANK/AY468396
SI  - GENBANK/AY468397
SI  - GENBANK/AY468398
SI  - GENBANK/AY468399
SI  - GENBANK/AY468400
SI  - GENBANK/AY468401
SI  - GENBANK/AY468402
SI  - GENBANK/AY468403
SI  - GENBANK/AY468404
SI  - GENBANK/AY468405
SI  - GENBANK/AY468406
SI  - GENBANK/AY468407
SI  - GENBANK/AY628413
SI  - GENBANK/AY628414
SI  - GENBANK/AY628415
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041115
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Codon)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Plant Proteins)
RN  - EC 3.2.1.39 (Glucan Endo-1,3-beta-D-Glucosidase)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Bayes Theorem
MH  - Codon
MH  - Conserved Sequence
MH  - Enzyme Inhibitors/chemistry/*pharmacology
MH  - Evolution, Molecular
MH  - Genes, Plant
MH  - Genetic Variation
MH  - Glucan Endo-1,3-beta-D-Glucosidase/*antagonists & inhibitors/drug 
      effects/*genetics
MH  - Models, Genetic
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Phytophthora/*enzymology
MH  - Plant Proteins/chemistry/*genetics/pharmacology
MH  - *Selection, Genetic
MH  - Sequence Homology, Amino Acid
MH  - Glycine max/enzymology
PMC - PMC1449112
EDAT- 2004/11/17 09:00
MHDA- 2005/07/16 09:00
PMCR- 2005/05/01
CRDT- 2004/11/17 09:00
PHST- 2004/11/17 09:00 [pubmed]
PHST- 2005/07/16 09:00 [medline]
PHST- 2004/11/17 09:00 [entrez]
PHST- 2005/05/01 00:00 [pmc-release]
AID - genetics.103.025098 [pii]
AID - 5098 [pii]
AID - 10.1534/genetics.103.025098 [doi]
PST - ppublish
SO  - Genetics. 2005 Feb;169(2):1009-19. doi: 10.1534/genetics.103.025098. Epub 2004 
      Nov 15.