PMID- 15547663
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20161124
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 14
IP  - 6
DP  - 2004 Dec
TI  - Investigation of MYCN status in neuroblastoma by fluorescence in situ 
      hybridization.
PG  - 981-7
AB  - Neuroblastoma, a form of neuroblastic tumor, is one of the most common neoplasms 
      seen in early childhood. The diverse clinical behavior of this tumor is most 
      probably explainable by the heterogeneity in the associated genetic changes. We 
      investigated 12 neuroblastoma patients for MYCN amplification and chromosome 2 
      aneusomy by fluorescence in situ hybridization (FISH) and results were correlated 
      with conventional cytogenetics. Samples from both primary tumor tissue and bone 
      marrow metastasis were available in two cases. The copy number of MYCN oncogene 
      paralleled that of chromosome 2 in 10 cases, whereas two cases (16.7%) showed 
      numerous distinct signals within the nuclei of the tumor cells, consistent with 
      MYCN amplification as double minute (dmin). The morphology of dmin in one case 
      was of an extremely small type and might potentially be missed by conventional 
      chromosome analysis. Discordant cytogenetics and FISH results was observed 
      between primary tumor and metastasis disease in one case, with loss of chromosome 
      2 tetrasomic and pentasomic cells as well as over-representation of chromosome 2 
      disomic cells harboring MYCN amplification in bone marrow deposits. Our study 
      reaffirmed the need for MYCN status to be determined in light of chromosome 2 
      copy number, as recommended by published guidelines. We also showed that genetic 
      heterogeneity might occur between primary tumor and bone marrow metastasis. 
      Finally, atypical dmin morphology when encountered would need confirmation by 
      FISH study.
FAU - Wan, Thomas S K
AU  - Wan TS
AD  - Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Hong 
      Kong SAR, P.R. China.
FAU - Ma, Edmond S K
AU  - Ma ES
FAU - Chan, Godfrey C F
AU  - Chan GC
FAU - Chan, L C
AU  - Chan LC
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (MYCN protein, human)
RN  - 0 (N-Myc Proto-Oncogene Protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Karyotyping
MH  - Male
MH  - N-Myc Proto-Oncogene Protein
MH  - Neuroblastoma/*genetics/pathology
MH  - Nuclear Proteins/*genetics
MH  - Oncogene Proteins/*genetics
EDAT- 2004/11/18 09:00
MHDA- 2005/04/29 09:00
CRDT- 2004/11/18 09:00
PHST- 2004/11/18 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2004/11/18 09:00 [entrez]
PST - ppublish
SO  - Int J Mol Med. 2004 Dec;14(6):981-7.