PMID- 15547936
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20061115
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 62
IP  - 4
DP  - 2005 Mar
TI  - CNS midline cells contribute to maintenance of the initial dorsoventral 
      patterning of the Drosophila ventral neuroectoderm.
PG  - 397-405
AB  - Dorsoventral patterning of the Drosophila ventral neuroectoderm is established by 
      the expression of three evolutionarily conserved homeodomain genes: ventral 
      nervous system defective (vnd), intermediate neuroblasts defective (ind), and 
      muscle segment homeobox (msh) in the medial, intermediate, and lateral columns of 
      the ventral neuroectoderm, respectively. It was not clear whether extrinsic 
      factor(s) from the CNS midline cells influence the initial dorsoventral 
      patterning by controlling the expression of the dorsoventral patterning genes. We 
      show here that the CNS midline cells, specified by single-minded (sim), are 
      essential for maintaining expression of the dorsoventral patterning genes. 
      Ectopic expression of sim in the ventral neuroectoderm during the blastoderm 
      stage repressed expression of the three homeodomain genes in the ventral 
      neuroectoderm. This indicates that the identity of the CNS midline cells is 
      established by a series of repressions of the three homeodomain genes in the 
      ventral neuroectoderm. Ectopic expression of sim in the ventral neuroectoderm 
      during initial neurogenesis induced ectopic ind expression in the medial column 
      in addition to that in the intermediate column via EGFR signaling between the 
      ventral neuroectoderm and midline cells. In contrast, it repressed the expression 
      of vnd and msh in the medial and lateral columns, respectively. Our findings 
      demonstrate that the CNS midline cells provide extrinsic positional information 
      via EGFR signaling that maintains the initial subdivision of the ventral 
      neuroectoderm into three dorsoventral columns during initial neurogenesis.
CI  - Copyright 2004 Wiley Periodicals, Inc.
FAU - Kim, In Ok
AU  - Kim IO
AD  - Department of Chemistry, Konkuk University, Seoul 143-701, Korea.
FAU - Kim, In Chul
AU  - Kim IC
FAU - Kim, Sunmi
AU  - Kim S
FAU - Kwon, Yeon Kyung
AU  - Kwon YK
FAU - Han, Pyung-Lim
AU  - Han PL
FAU - Jeon, Sang-Hak
AU  - Jeon SH
FAU - Kim, Sang Hee
AU  - Kim SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Drosophila Proteins)
SB  - IM
MH  - Animals
MH  - Central Nervous System/*cytology/embryology/metabolism
MH  - Drosophila/*cytology/embryology/metabolism
MH  - Drosophila Proteins/biosynthesis
MH  - Ectoderm/*cytology/metabolism
MH  - Gene Expression Regulation, Developmental/physiology
EDAT- 2004/11/18 09:00
MHDA- 2005/04/29 09:00
CRDT- 2004/11/18 09:00
PHST- 2004/11/18 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2004/11/18 09:00 [entrez]
AID - 10.1002/neu.20104 [doi]
PST - ppublish
SO  - J Neurobiol. 2005 Mar;62(4):397-405. doi: 10.1002/neu.20104.