PMID- 15548530
OWN - NLM
STAT- MEDLINE
DCOM- 20050513
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 280
IP  - 13
DP  - 2005 Apr 1
TI  - Jingzhaotoxin-I, a novel spider neurotoxin preferentially inhibiting cardiac 
      sodium channel inactivation.
PG  - 12069-76
AB  - Jingzhaotoxin-I (JZTX-I), a 33-residue polypeptide, is derived from the Chinese 
      tarantula Chilobrachys jing-zhao venom based on its ability to evidently increase 
      the strength and the rate of vertebrate heartbeats. The toxin has three disulfide 
      bonds with the linkage of I-IV, II-V, and III-VI that is a typical pattern found 
      in inhibitor cystine knot molecules. Its cDNA determined by rapid amplification 
      of 3'- and 5'-cDNA ends encoded a 62-residue precursor with a small proregion of 
      eight residues. Whole-cell configuration indicated that JZTX-I was a novel 
      neurotoxin preferentially inhibiting cardiac sodium channel inactivation by 
      binding to receptor site 3. Although JZTX-I also exhibits the interaction with 
      channel isoforms expressing in mammalian and insect sensory neurons, its affinity 
      for tetrodotoxin-resistant subtype in mammalian cardiac myocytes (IC50 = 31.6 nm) 
      is approximately 30-fold higher than that for tetrodotoxin-sensitive subtypes in 
      latter tissues. Not affecting outward delay-rectified potassium channels 
      expressed in Xenopus laevis oocytes and tetrodotoxin-resistant sodium channels in 
      mammal sensory neurons, JZTX-I hopefully represents a potent ligand to 
      discriminate cardiac sodium channels from neuronal tetrodotoxin-resistant 
      isoforms. Furthermore, different from any reported spider toxins, the toxin 
      neither modifies the current-voltage relationships nor shifts the steady-state 
      inactivation of sodium channels. Therefore, JZTX-I defines a new subclass of 
      spider sodium channel toxins. JZTX-I is an alpha-like toxin first reported from 
      spider venoms. The result provides an important witness for a convergent 
      functional evolution between spider and other animal venoms.
FAU - Xiao, Yucheng
AU  - Xiao Y
AD  - College of Life Sciences, Hunan Normal University, Changsha 410081, China.
FAU - Tang, Jianzhou
AU  - Tang J
FAU - Hu, Weijun
AU  - Hu W
FAU - Xie, Jinyun
AU  - Xie J
FAU - Maertens, Chantal
AU  - Maertens C
FAU - Tytgat, Jan
AU  - Tytgat J
FAU - Liang, Songping
AU  - Liang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041117
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Disulfides)
RN  - 0 (Ligands)
RN  - 0 (Neurotoxins)
RN  - 0 (Peptides)
RN  - 0 (Potassium Channels)
RN  - 0 (Protein Isoforms)
RN  - 0 (Sodium Channel Blockers)
RN  - 0 (Sodium Channels)
RN  - 0 (Spider Venoms)
RN  - 0 (jingzhaotoxin-I, Chilobrachys jingzhao)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/metabolism
MH  - Disulfides/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Evolution, Molecular
MH  - Female
MH  - Inhibitory Concentration 50
MH  - Insecta
MH  - Ligands
MH  - Male
MH  - Membrane Potentials
MH  - Molecular Sequence Data
MH  - Myocardium/metabolism
MH  - Myocytes, Cardiac/drug effects/metabolism
MH  - Neurons/drug effects/metabolism
MH  - Neurotoxins/chemistry
MH  - Oocytes/drug effects/metabolism
MH  - Peptides/*chemistry/pharmacology
MH  - Phylogeny
MH  - Potassium Channels/chemistry
MH  - Protein Isoforms
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sodium Channel Blockers/*pharmacology
MH  - Sodium Channels/*chemistry/metabolism
MH  - Spider Venoms/*chemistry/pharmacology
MH  - Spiders
MH  - Tetrodotoxin/chemistry
MH  - Time Factors
MH  - Xenopus laevis
EDAT- 2004/11/19 09:00
MHDA- 2005/05/14 09:00
CRDT- 2004/11/19 09:00
PHST- 2004/11/19 09:00 [pubmed]
PHST- 2005/05/14 09:00 [medline]
PHST- 2004/11/19 09:00 [entrez]
AID - S0021-9258(19)60528-4 [pii]
AID - 10.1074/jbc.M411651200 [doi]
PST - ppublish
SO  - J Biol Chem. 2005 Apr 1;280(13):12069-76. doi: 10.1074/jbc.M411651200. Epub 2004 
      Nov 17.