PMID- 15548678
OWN - NLM
STAT- MEDLINE
DCOM- 20050224
LR  - 20161124
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 64
IP  - 22
DP  - 2004 Nov 15
TI  - Cul4A physically associates with MDM2 and participates in the proteolysis of p53.
PG  - 8152-5
AB  - The cullin 4A (Cul4A) gene is amplified and overexpressed in breast and 
      hepatocellular carcinomas. Cul4A functions as an E3 ligase and participates in 
      the proteolysis of several regulatory proteins through the ubiquitin-proteasome 
      pathway. Here, we show that Cul4A associates with MDM2 and p53. Depletion of 
      Cul4A leads to an accumulation of p53. Moreover, expression of Cul4A increases 
      the decay-rate of p53 and delays the accumulation of p53 in response to DNA 
      damage. Cul4A fails to increase the decay of p53 in mouse embryonic fibroblasts 
      lacking MDM2. In addition, the Cul4A-mediated rapid decay of p53 is blocked by 
      p19ARF. The results provide evidence for a role of Cul4A in the MDM2-mediated 
      proteolysis of p53.
FAU - Nag, Alo
AU  - Nag A
AD  - Department of Biochemistry and Molecular Genetics, College of Dentistry, 
      University of Illinois at Chicago, Chicago, Illinois 60607, USA.
FAU - Bagchi, Srilata
AU  - Bagchi S
FAU - Raychaudhuri, Pradip
AU  - Raychaudhuri P
LA  - eng
GR  - CA88863/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (CUL4A protein, human)
RN  - 0 (Cullin Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - Cullin Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Hydrolysis
MH  - Nuclear Proteins/*metabolism
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-mdm2
MH  - Tumor Suppressor Protein p53/*metabolism
EDAT- 2004/11/19 09:00
MHDA- 2005/02/25 09:00
CRDT- 2004/11/19 09:00
PHST- 2004/11/19 09:00 [pubmed]
PHST- 2005/02/25 09:00 [medline]
PHST- 2004/11/19 09:00 [entrez]
AID - 64/22/8152 [pii]
AID - 10.1158/0008-5472.CAN-04-2598 [doi]
PST - ppublish
SO  - Cancer Res. 2004 Nov 15;64(22):8152-5. doi: 10.1158/0008-5472.CAN-04-2598.