PMID- 15555924
OWN - NLM
STAT- MEDLINE
DCOM- 20050302
LR  - 20141120
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 27
IP  - 4
DP  - 2004 Dec
TI  - Estrogen deficiency leads to apoptosis in dopaminergic neurons in the medial 
      preoptic area and arcuate nucleus of male mice.
PG  - 466-76
AB  - The aromatase knockout (ArKO) mouse is unable to synthesize estrogens. 
      Immunohistochemical studies on active caspase-3 and tyrosine hydroxylase (TH) 
      revealed apoptosis of dopaminergic neurons in the medial preoptic area (MPO) and 
      arcuate nucleus (Arc) of the hypothalamus of 1-year-old (1yo) male ArKO mice 
      while no active caspase-3 was detected in wild type (WT). Furthermore, the number 
      of TH-positive cells in the MPO and caudal Arc was significantly decreased in 1yo 
      ArKO compared to WT. RNase protection assays support the presence of apoptosis in 
      1yo ArKO hypothalamus, revealing an up-regulation of pro-apoptotic genes: FASL, 
      FADD, and caspase-8. Concomitantly, the ratio of bcl-2-related anti-apoptotic 
      genes to pro-apoptotic genes in the hypothalamus of 1yo ArKO mice was 
      significantly down-regulated. Previously, we have reported that no such changes 
      were observed in the hypothalamus of female ArKO mice. Thus, we have provided 
      direct evidence that estrogen is required to maintain the survival and functional 
      integrity of dopaminergic neurons in the MPO and Arc of male, but not female 
      mice.
FAU - Hill, Rachel A
AU  - Hill RA
AD  - Prince Henry's Institute of Medical Research, Clayton 3168, VIC, Australia.
FAU - Pompolo, Sueli
AU  - Pompolo S
FAU - Jones, Margaret E E
AU  - Jones ME
FAU - Simpson, Evan R
AU  - Simpson ER
FAU - Boon, Wah Chin
AU  - Boon WC
LA  - eng
GR  - R37AG08174/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Estrogens)
RN  - 0 (Fadd protein, mouse)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Casp8 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspases)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Arcuate Nucleus of Hypothalamus/pathology/*physiopathology
MH  - Aromatase/deficiency/genetics
MH  - Caspase 3
MH  - Caspase 8
MH  - Caspases/genetics/metabolism
MH  - Cell Count
MH  - Cell Survival/genetics
MH  - Dopamine/*metabolism
MH  - Down-Regulation/genetics
MH  - Estrogens/biosynthesis/*deficiency
MH  - Fas Ligand Protein
MH  - Fas-Associated Death Domain Protein
MH  - Genes, bcl-2/genetics
MH  - Male
MH  - Membrane Glycoproteins/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Degeneration/genetics/*metabolism
MH  - Preoptic Area/pathology/*physiopathology
MH  - RNA, Messenger/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Up-Regulation/genetics
EDAT- 2004/11/24 09:00
MHDA- 2005/03/03 09:00
CRDT- 2004/11/24 09:00
PHST- 2004/01/08 00:00 [received]
PHST- 2004/03/24 00:00 [revised]
PHST- 2004/04/27 00:00 [accepted]
PHST- 2004/11/24 09:00 [pubmed]
PHST- 2005/03/03 09:00 [medline]
PHST- 2004/11/24 09:00 [entrez]
AID - S1044-7431(04)00174-5 [pii]
AID - 10.1016/j.mcn.2004.04.012 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2004 Dec;27(4):466-76. doi: 10.1016/j.mcn.2004.04.012.