PMID- 15556534
OWN - NLM
STAT- MEDLINE
DCOM- 20050411
LR  - 20061115
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 813
IP  - 1-2
DP  - 2004 Dec 25
TI  - Determination of underivatised sterols and bile acid trimethyl silyl ether methyl 
      esters by gas chromatography-mass spectrometry-single ion monitoring in faeces.
PG  - 199-207
AB  - A method for quantification of total faecal sterols and bile acids (BAs) in human 
      stool by using gas chromatography-mass spectrometry-single ion monitoring 
      (GC-MS-SIM) is described. Cholesterol, coprostanol, coprostanone, cholestanol, 
      iso-lithocholic acid (iso-LCA), lithocholic acid (LCA), iso-deoxycholic acid 
      (iso-DCA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), cholic acid 
      (CA), and 12-oxo-deoxycholic acid (12-oxo-DCA) in faeces of 86 healthy subjects 
      were determined. The sample preparation for sterol analysis requires hydrolysis 
      and liquid extraction from matrix, but no derivatisation. The GC-flame ionisation 
      detection (FID) and total ion current (TIC) in GC-MS were not sufficient for 
      sterol and BA determination, whereas selectivity and specificity of the GC-MS-SIM 
      ensured the analysis of sterols and BAs in faeces.
FAU - Keller, Sylvia
AU  - Keller S
AD  - Friedrich Schiller University, Institute of Nutrition, Dornburger Str. 24, 07743 
      Jena, Germany.
FAU - Jahreis, Gerhard
AU  - Jahreis G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Esters)
RN  - 0 (Sterols)
SB  - IM
MH  - Bile Acids and Salts/*analysis/chemistry
MH  - Calibration
MH  - Esters/analysis
MH  - Feces/*chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Reproducibility of Results
MH  - Sterols/*analysis
EDAT- 2004/11/24 09:00
MHDA- 2005/04/12 09:00
CRDT- 2004/11/24 09:00
PHST- 2003/03/13 00:00 [received]
PHST- 2004/09/21 00:00 [accepted]
PHST- 2004/11/24 09:00 [pubmed]
PHST- 2005/04/12 09:00 [medline]
PHST- 2004/11/24 09:00 [entrez]
AID - S1570-0232(04)00781-0 [pii]
AID - 10.1016/j.jchromb.2004.09.046 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):199-207. doi: 
      10.1016/j.jchromb.2004.09.046.