PMID- 15556679
OWN - NLM
STAT- MEDLINE
DCOM- 20051017
LR  - 20041123
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 65
IP  - 11
DP  - 2004 Nov
TI  - Detection and specification of noncomplement binding anti-HLA alloantibodies.
PG  - 1288-96
AB  - The aim of the study was to investigate the distribution of human leukocyte 
      antigen (HLA) -specific immunoglobulin (Ig) isotypes/subclasses in alloimmunized 
      patients awaiting a kidney retransplant. Sera from 102 patients were analyzed for 
      the presence of anti-HLA-A, anti-HLA-B alloantibodies by complement-dependent 
      cytotoxicity test with the addition of dithiothreitol (CDC+DTT). Furthermore, 
      anti-HLA class I and class II alloantibodies were determined using a commercial 
      solid-phase (enzyme-linked immunosorbent assay [ELISA]) system. The respective 
      isotypes/subclasses were defined by replacing the IgG1-4 secondary antibody with 
      IgG1-, IgG2-, IgG3-, IgG4-, IgA1-, IgA2-, and IgM-specific antibodies. The HLA 
      specificities of the noncomplement-binding IgG2 and IgG4 antibodies were 
      determined and compared with the mismatches from the failed transplants. 
      Thirty-eight of 102 (37%) sera were positive in the class I CDC+DTT, in contrast 
      to 41 of 102 (40%) detected by class I ELISA and 47 of 102 (46%) by class II 
      ELISA. Seventeen of 102 (17%) positive reaction were observed for the 
      IgM-isotype, whereas none were detected for the IgA-isotype. Twenty-five of 102 
      (25 %) sera contained noncomplement-binding IgG2 and/or IgG4 antibodies; in the 
      majority of the cases, 22 of 25 (88%) were directed against the organ donor 
      antigen. These data show that donor-specific, noncomplement-binding IgG2 and IgG4 
      alloantibodies exist with high prevalence in HLA-immunized retransplant 
      candidates. Therefore, a thorough antibody screening workup, including CDC with 
      or without DTT and ELISA screening should be performed for patients before they 
      reenter the waiting list. Defining the Ig isotypes and subclasses can be helpful 
      to explain inconsistent results.
FAU - Arnold, Marie-Luise
AU  - Arnold ML
AD  - Institute for Clinical Immunology and Department of Medicine, University of 
      Erlangen-Nuremberg, Erlangen-Nuremberg, Germany. 
      marlies.arnold@med3.imed.uni-erlangen.de
FAU - Zacher, Thorsten
AU  - Zacher T
FAU - Dechant, Michael
AU  - Dechant M
FAU - Kalden, Joachim R
AU  - Kalden JR
FAU - Doxiadis, Ilias I N
AU  - Doxiadis II
FAU - Spriewald, Bernd M
AU  - Spriewald BM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Antibody Specificity/*immunology
MH  - HLA Antigens/*immunology
MH  - HLA-A Antigens/immunology
MH  - HLA-B Antigens/immunology
MH  - HLA-D Antigens/immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Immunoglobulin A/blood/immunology
MH  - Immunoglobulin G/blood/immunology
MH  - Immunoglobulin M/blood/immunology
MH  - Isoantibodies/*blood/immunology
MH  - Kidney Transplantation/immunology
MH  - Protein Binding
MH  - Time Factors
EDAT- 2004/11/24 09:00
MHDA- 2005/10/18 09:00
CRDT- 2004/11/24 09:00
PHST- 2004/07/05 00:00 [received]
PHST- 2004/07/29 00:00 [revised]
PHST- 2004/08/24 00:00 [accepted]
PHST- 2004/11/24 09:00 [pubmed]
PHST- 2005/10/18 09:00 [medline]
PHST- 2004/11/24 09:00 [entrez]
AID - S0198-8859(04)00605-6 [pii]
AID - 10.1016/j.humimm.2004.08.182 [doi]
PST - ppublish
SO  - Hum Immunol. 2004 Nov;65(11):1288-96. doi: 10.1016/j.humimm.2004.08.182.