PMID- 15560921
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20151119
IS  - 0165-022X (Print)
IS  - 0165-022X (Linking)
VI  - 61
IP  - 1-2
DP  - 2004 Oct 29
TI  - HPLC-RIA analysis of steroid hormone profile in a virilizing stromal tumor of the 
      ovary.
PG  - 47-56
AB  - The pathological steroid biosynthesis of a virilizing ovarian tumor was examined 
      via high performance liquid chromatography-radioimmunoassay (HPLC-RIA) 
      determination of the intratissular steroid concentrations. Sex cord-stromal tumor 
      of the ovary was obtained surgically from an 18-year-old female patient with 
      extremely high androst-4-ene-3,17-dione (4-en-dione) and testosterone (Test) 
      blood serum levels. The tissue specimen was extracted with ethyl acetate and the 
      extract was then purified on a C18 mini-column with methanol-water eluents. 
      Steroids were isolated by reversed-phase HPLC on a C18 silica gel column with 
      51%, 55% and 64% v/v methanol-water eluents. Steroids in the collected eluent 
      fractions were detected by the radioactivity of tritiated internal standards and 
      then quantified by specific RIAs. In the tumor specimen, very high 
      17alpha-hydroxyprogesterone (17-OH-Prog; 6300 fmol/g), dehydro-epiandrosterone 
      (2870 fmol/g), androst-4-ene-3,17-dione (3000 fmol/g), testosterone (5700 fmol/g) 
      concentrations, and less progesterone (PROG; 320 fmol/g) and 
      androst-5-ene-3beta,17beta-diol (5-en-diol; 320 fmol/g), were determined. Tissue 
      levels of 5alpha-dihydrotestosterone (DHT), 5alpha-androstane-3alpha,17beta-diol 
      (3alpha-diol), 5alpha-androstane-3beta,17beta-diol (3beta-diol), and 
      17beta-estradiol were found to be 71, 20, 28, and 12 fmol/g, respectively. 
      Steroid profile analysis verified a pathological steroid biosynthesis in the 
      ovarian tumor and suggested that the 17alpha-hydroxylase (17alpha-H), 17,20-lyase 
      (17,20-L), and 3beta-hydroxysteroid dehydrogenase/Delta5-4-isomerase 
      (Delta5-3beta-HSD) activities were particularly elevated in this tumorous tissue. 
      Present data demonstrate that the analysis of intratissular steroid profile by a 
      HPLC-RIA method may valuably contribute to the steroidal pathophysiology of 
      endocrine tumors.
FAU - Szecsi, M
AU  - Szecsi M
AD  - Endocrine Unit and Research Laboratory, University of Szeged, 8 Koranyi fasor, 
      H-6720 Szeged, Hungary. szecsim@endoc.szote.u-szeged.hu
FAU - Toth, I
AU  - Toth I
FAU - Gardi, J
AU  - Gardi J
FAU - Nyari, T
AU  - Nyari T
FAU - Julesz, J
AU  - Julesz J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Biochem Biophys Methods
JT  - Journal of biochemical and biophysical methods
JID - 7907378
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Gonadal Steroid Hormones)
SB  - IM
MH  - Adolescent
MH  - Biomarkers, Tumor/*metabolism
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Endometrial Stromal Tumors/complications/*metabolism
MH  - Female
MH  - Gonadal Steroid Hormones/*analysis/*metabolism
MH  - Humans
MH  - Ovarian Neoplasms/complications/*metabolism
MH  - Radioimmunoassay/*methods
MH  - Tumor Cells, Cultured
MH  - Virilism/etiology/*metabolism
EDAT- 2004/11/25 09:00
MHDA- 2005/04/22 09:00
CRDT- 2004/11/25 09:00
PHST- 2003/11/04 00:00 [received]
PHST- 2004/04/07 00:00 [revised]
PHST- 2004/04/09 00:00 [accepted]
PHST- 2004/11/25 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2004/11/25 09:00 [entrez]
AID - S0165022X04000648 [pii]
AID - 10.1016/j.jbbm.2004.04.008 [doi]
PST - ppublish
SO  - J Biochem Biophys Methods. 2004 Oct 29;61(1-2):47-56. doi: 
      10.1016/j.jbbm.2004.04.008.