PMID- 15561097
OWN - NLM
STAT- MEDLINE
DCOM- 20050111
LR  - 20061115
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 302
IP  - 2
DP  - 2005 Jan 15
TI  - The use of non-physiological conditions to isolate fetal cells from maternal 
      blood.
PG  - 153-61
AB  - Fetal cells are always present in maternal blood starting in the first trimester 
      of pregnancy, however a rapid, simple, and consistent procedure for their 
      isolation for prenatal non-invasive genetic investigation is still lacking. 
      Sensitivity and recovery of fetal cells is jeopardized by the minute amount of 
      circulating fetal cells and their loss during the enrichment procedure. We report 
      here a single-step approach to isolate fetal cells from maternal blood which 
      relies on the use of non-physiological conditions to modify cell densities before 
      their separation in a density gradient and in a newly developed cell separation 
      device. Isolated fetal cells have been investigated using cytochemistry, Soret 
      band absorption microscopy, monoclonal antibodies for epsilon- and 
      gamma-chain-Hb, monoclonal antibody for i-antigen, and by fluorescence in situ 
      hybridization (FISH). Fetal cells were always detected in all 105 maternal blood 
      samples investigated and fetal aneuploidies were correctly diagnosed by FISH, in 
      a pilot study of pathological pregnancies, in fetal cells isolated from maternal 
      blood obtained either before or after invasive procedure.
FAU - Sitar, Giammaria
AU  - Sitar G
AD  - Department of Medicine Policlinico San Matteo and University of Pavia, Italy. 
      g.sitar@smatteo.pv.it
FAU - Brambati, Bruno
AU  - Brambati B
FAU - Baldi, Marina
AU  - Baldi M
FAU - Montanari, Laura
AU  - Montanari L
FAU - Vincitorio, Massimo
AU  - Vincitorio M
FAU - Tului, Lucia
AU  - Tului L
FAU - Forabosco, Antonino
AU  - Forabosco A
FAU - Ascari, Edoardo
AU  - Ascari E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antibodies, Monoclonal)
RN  - 9034-63-3 (Fetal Hemoglobin)
SB  - IM
MH  - Aneuploidy
MH  - Antibodies, Monoclonal/metabolism
MH  - Blood Cells/*cytology
MH  - Cell Separation/*methods
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Fetal Diseases/pathology
MH  - Fetal Hemoglobin/metabolism
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pilot Projects
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Prenatal Diagnosis/*methods
EDAT- 2004/11/25 09:00
MHDA- 2005/01/12 09:00
CRDT- 2004/11/25 09:00
PHST- 2004/03/17 00:00 [received]
PHST- 2004/07/24 00:00 [revised]
PHST- 2004/11/25 09:00 [pubmed]
PHST- 2005/01/12 09:00 [medline]
PHST- 2004/11/25 09:00 [entrez]
AID - S0014-4827(04)00516-6 [pii]
AID - 10.1016/j.yexcr.2004.08.039 [doi]
PST - ppublish
SO  - Exp Cell Res. 2005 Jan 15;302(2):153-61. doi: 10.1016/j.yexcr.2004.08.039.