PMID- 15561266
OWN - NLM
STAT- MEDLINE
DCOM- 20050505
LR  - 20211203
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 36
IP  - 8
DP  - 2004 Oct
TI  - CD8 T cell of donor splenocyte mixed with bone marrow cells is more effective 
      than CD4 T cell for induction of donor-specific tolerance in sublethally 
      irradiated mice.
PG  - 2418-22
AB  - BACKGROUND: We previously demonstrated that even a low dose of bone marrow cells 
      (BMCs) established donor-specific tolerance if mixed with splenocytes (SPLCs). In 
      this study, T-cell subsets CD4 (CD4SP) and CD8 (CD8SP) of donor SPLCs were 
      investigated for their contribution to the enhancement of BMC engraftment leading 
      to donor-specific tolerance in sublethally irradiated mice. METHODS: Sublethally 
      irradiated C57BL/6 recipient mice were intravenously injected BMCs mixing with 
      CD4SP or CD8SP harvested from BALB/c donor mice. The degree of chimerism in the 
      peripheral blood lymphocytes (PBLs) and in the SPLCs was analyzed using FACS, 
      mixed lymphocyte reaction, and skin graft transplantation 3 months after 
      injection. RESULTS: Recipients injected with 3 x 10(6) donor BMCs admixed with 10 
      x 10(6) donor CD8SP established chimerism. However, recipients injected with the 
      same dose of BMCs admixed with 5 x 10(6) CD4SP, 10 x 10(6) CD4SP, and 5 x 10(6) 
      CD8SP did not established chimerism. CD8SP contained 44% of Ly6A/E (Stem Cell 
      Antigen-1 (Sca-1))-positive cells based on FACS analysis, whereas only 6% of 
      CD4SP were positive for Ly6A/E. MLR supernates of donor SPLCs chimeric mice using 
      admixture with CD8SP dominated by Th2 cytokines. In contrast, mixting with MLR 
      supernates from failed chimera showed dominant Th1 cytokines. CONCLUSIONS: CD8SP 
      seems to make a major contribution to enhance BMC engraftment and induce 
      donor-specific tolerance. Ly6A/E (Sca-1)-positive cells need to be further 
      investigated for their contribution to the establishment of chimerism.
FAU - Yoshida, A
AU  - Yoshida A
AD  - Department of Surgery, Hirosaki University School of Medicine, Hirosaki, Japan.
FAU - Narumi, S
AU  - Narumi S
FAU - Hashimoto, N
AU  - Hashimoto N
FAU - Itabashi, Y
AU  - Itabashi Y
FAU - Hakamada, K
AU  - Hakamada K
FAU - Sasaki, M
AU  - Sasaki M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cytokines/immunology
MH  - Female
MH  - Graft Survival/immunology
MH  - Immunosuppression Therapy/methods
MH  - Lymphocyte Culture Test, Mixed
MH  - *Lymphocyte Transfusion
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Skin Transplantation/*immunology
MH  - Spleen/immunology
MH  - T-Lymphocyte Subsets/immunology
MH  - Whole-Body Irradiation
EDAT- 2004/11/25 09:00
MHDA- 2005/05/06 09:00
CRDT- 2004/11/25 09:00
PHST- 2004/11/25 09:00 [pubmed]
PHST- 2005/05/06 09:00 [medline]
PHST- 2004/11/25 09:00 [entrez]
AID - S0041-1345(04)00891-7 [pii]
AID - 10.1016/j.transproceed.2004.08.028 [doi]
PST - ppublish
SO  - Transplant Proc. 2004 Oct;36(8):2418-22. doi: 10.1016/j.transproceed.2004.08.028.