PMID- 15565683
OWN - NLM
STAT- MEDLINE
DCOM- 20050419
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 25
IP  - 21-22
DP  - 2004 Nov
TI  - Direct electrochemical detection of glucose in human plasma on capillary 
      electrophoresis microchips.
PG  - 3853-9
AB  - We developed an electrochemical detector on a hybrid chip for the determination 
      of glucose in human plasma. The microchip system described in this paper consists 
      of a poly(dimethylsiloxane) (PDMS) layer containing separation and injection 
      channels and an electrode plate. The copper microelectrode is fabricated by 
      selective electroless deposition. The fabrication of the decoupler is performed 
      by platinum electrochemical deposition on the metal film formed by electroless 
      deposition. Factors influencing the performance, including detection potential, 
      separation field strength, and buffer concentration, were studied. The electrodes 
      exhibited good stability and durability in the analytical procedures. Under 
      optimized detection conditions, glucose responded linearly from 10 microM to 1 
      mM. Finally, glucose in human plasma from three healthy individuals and two 
      diabetics was successfully determined, giving a good prospect for a new clinical 
      diagnostic instrument.
FAU - Du, Yan
AU  - Du Y
AD  - College of Chemistry, Jilin University, Changchun, China.
FAU - Yan, Jilin
AU  - Yan J
FAU - Zhou, Weihong
AU  - Zhou W
FAU - Yang, Xiuyong
AU  - Yang X
FAU - Wang, Erkang
AU  - Wang E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Blood Glucose)
RN  - 0 (Buffers)
SB  - IM
MH  - Blood Glucose/*analysis
MH  - Buffers
MH  - Diabetes Mellitus/blood/diagnosis
MH  - Electrochemistry
MH  - Electrodes
MH  - Electrophoresis, Microchip/*methods/standards
MH  - Equipment Design
MH  - Humans
MH  - Sensitivity and Specificity
EDAT- 2004/11/27 09:00
MHDA- 2005/04/20 09:00
CRDT- 2004/11/27 09:00
PHST- 2004/11/27 09:00 [pubmed]
PHST- 2005/04/20 09:00 [medline]
PHST- 2004/11/27 09:00 [entrez]
AID - 10.1002/elps.200406072 [doi]
PST - ppublish
SO  - Electrophoresis. 2004 Nov;25(21-22):3853-9. doi: 10.1002/elps.200406072.