PMID- 15566185
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 50
IP  - 8
DP  - 2004
TI  - In-situ characterization of microbial community in an A/O submerged membrane 
      bioreactor with nitrogen removal.
PG  - 41-8
AB  - The bacterial community involved in removing nitrogen from sewage and their 
      preferred DO environment within an anoxic/oxic membrane bioreactor (A/O MBR) was 
      investigated. A continuously operated laboratory-scale A/O MBR was maintained for 
      360 d. At a sludge age of 150 d and a C/N ratio of 3.5, the system was capable of 
      removing 88% of the influent nitrogen from raw wastewater through typical 
      nitrogen removal transformations (i.e. aerobic ammonia oxidation and anoxic 
      nitrate reduction). Characterization of the A/O MBR bacterial community was 
      carried out using fluorescence in situ hybridization (FISH) techniques. FISH 
      results further showed that Nitrosospira spp. and Nitrospira spp. were the 
      predominant groups of ammonia and nitrite oxidizing group, respectively. They 
      constituted up to 11% and 6% of eubacteria at DO below 0.05 mg/l (low DO), 
      respectively, and about 14% and 9% of eubacteria at DO between 2-5 mg/l 
      (sufficient DO), respectively, indicating preference of nitrifiers for a higher 
      DO environment. Generally low counts of the genus Paracoccus were detected while 
      negative results were observed for Paracoccus denitrificans, Alcaligenes spp, and 
      Pseudomonas stutzeri under the low and sufficient DO environments. The overall 
      results indicate that Nitrosospira spp., Nitrospira spp. and members of 
      Paracoccus spp. can be metabolically functional in nitrogen removal in the 
      laboratory-scale A/O MBR system.
FAU - Sofia, A
AU  - Sofia A
AD  - Centre for Water Research, Department of Civil Engineering, National University 
      of Singapore, Blk E1A, Singapore.
FAU - Liu, W T
AU  - Liu WT
FAU - Ong, S L
AU  - Ong SL
FAU - Ng, W J
AU  - Ng WJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Nitrites)
RN  - 0 (Sewage)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Alcaligenes/genetics/isolation & purification/metabolism
MH  - *Bioreactors
MH  - *In Situ Hybridization, Fluorescence
MH  - Membranes
MH  - Nitrites/metabolism
MH  - Nitrogen/*isolation & purification/metabolism
MH  - Paracoccus denitrificans/genetics/isolation & purification/metabolism
MH  - Pseudomonas stutzeri/genetics/isolation & purification/metabolism
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid/*methods
EDAT- 2004/11/30 09:00
MHDA- 2005/04/22 09:00
CRDT- 2004/11/30 09:00
PHST- 2004/11/30 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2004/11/30 09:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2004;50(8):41-8.