PMID- 15566410
OWN - NLM
STAT- MEDLINE
DCOM- 20050422
LR  - 20041129
IS  - 0914-3505 (Print)
IS  - 0914-3505 (Linking)
VI  - 44
IP  - 4
DP  - 2004 Dec
TI  - Studies on preliminary concentration methods for recovery of fetal nucleated red 
      blood cells in maternal blood.
PG  - 196-203
AB  - Non-invasive prenatal diagnostic methods posing no danger to the embryo have been 
      desired for many years in the field of prenatal medicine. We are in the process 
      of improving the lectin method that we developed for recovering fetus-derived 
      nucleated red blood cells (NRBC) in the maternal peripheral blood. We previously 
      used Ficoll density-gradient centrifugation for preliminary concentration in the 
      lectin method. In the present study, we developed a molecular filter method, 
      compared it with the Ficoll method, and tested its applicability to prenatal 
      diagnosis. We tested the usefulness of a high molecular filter method for 
      preliminary concentration. First, in a basic study, we prepared three kinds of 
      non-woven cloth (NWC1-3) and a multi-porous filter (MP) to determine the optimal 
      filter. Next, we compared the recovery rates of the Ficoll and filter methods as 
      preliminary concentration methods in 34 normal pregnant women. Then, to examine 
      whether the recovered NRBC were derived from the fetus, we attempted prenatal 
      diagnosis by the fluorescence in situ hybridization (FISH) technique in 12 women 
      pregnant with a male fetus (determined later by ultrasound) at between 8 and 16 
      weeks of gestation. Among the four devices used in the basic study of the high 
      molecular filter method, NWC-2 had the best recovery rate. Therefore, we compared 
      the numbers of NRBC recovered by the lectin method after preliminary 
      concentration with NWC-2 or by Ficoll centrifugation, and found that the mean 
      recovery rate of NWC-2 was 4.2 +/- 5.0 times as high as that of the Ficoll 
      method, indicating that the NWC-2 filter method is superior as a preliminary 
      concentration method. Next, FISH analysis of the 12 pregnant women with a male 
      fetus for the Y chromosome showed that 19.5 +/- 12.8 NRBC were recovered, in 12.7 
      +/- 8.1 (63.6%) of which a Y signal was confirmed, suggesting the NWC-2 filter 
      method can be applied to prenatal diagnosis. We consider the filter-lectin method 
      to be a superior method for isolation and recovery of NRBC in the maternal blood 
      which can be applied to prenatal diagnosis.
FAU - Wachi, Toshiki
AU  - Wachi T
AD  - Department of Obstetrics and Gynecology, The Jikei University School of Medicine, 
      3-25-8 Nishi-shinbashi, Minato-ku, Tokyo, Japan. wachi-t@ncchd.go.jp
FAU - Kitagawa, Michihiro
AU  - Kitagawa M
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Congenit Anom (Kyoto)
JT  - Congenital anomalies
JID - 9306292
SB  - IM
MH  - Cell Nucleus
MH  - Erythroblasts/*cytology
MH  - Female
MH  - *Fetus
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Prenatal Diagnosis/methods
EDAT- 2004/11/30 09:00
MHDA- 2005/04/23 09:00
CRDT- 2004/11/30 09:00
PHST- 2004/11/30 09:00 [pubmed]
PHST- 2005/04/23 09:00 [medline]
PHST- 2004/11/30 09:00 [entrez]
AID - CGA040 [pii]
AID - 10.1111/j.1741-4520.2004.00040.x [doi]
PST - ppublish
SO  - Congenit Anom (Kyoto). 2004 Dec;44(4):196-203. doi: 
      10.1111/j.1741-4520.2004.00040.x.