PMID- 15566835
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20050324
LR  - 20191109
IS  - 0928-0197 (Print)
IS  - 0928-0197 (Linking)
VI  - 4
IP  - 2
DP  - 1995 Aug
TI  - Antibody reactivity to individual structural proteins of measles virus in the CSF 
      of SSPE and MS patients.
PG  - 135-47
AB  - BACKGROUND: Chronic progressive disorders of the central nervous system (CNS) 
      impose diagnostic problems, particularly in younger patients. The demonstration 
      of antibodies against measles virus (MV) in the cerebrospinal fluid (CSF) plays a 
      major role in the laboratory diagnosis of subacute sclerosing panencephalitis 
      (SSPE) as well as multiple sclerosis (MS). OBJECTIVES: Because intrathecally 
      synthesized antibodies against MV can be found in both diseases, it is necessary 
      to establish easy and reliable methods to improve the differential diagnosis. 
      STUDY DESIGN: Seventy-one paired serum/CSF samples obtained from patients with 
      the diagnosis of SSPE (n = 23), MS (n = 14), or acute postinfectious measles 
      encephalitis (APME, n = 8) have been examined. The reactivity of intrathecally 
      synthesized immunoglobulin to individual recombinant MV structural proteins was 
      assessed using Western blot analysis, ELISA as well as isoelectric focusing 
      (IEF). RESULTS: All CSF samples obtained from patients suffering from SSPE showed 
      a strong antibody response to MV-nucleocapsid (N) and phosphoprotein (P). Sera 
      from 15 of the 23 SSPE patients were reactive to MV-fusion protein (F). Faint 
      reactivity was obtained against MV-matrix (M) or hemagglutinin protein (H) in the 
      minority of samples (40 and 20%, respectively). CSF samples of MS patients only 
      revealed a clear response to N, and in two cases to F. The other proteins were 
      not recognized in the CSF samples of MS patients. In contrast to SSPE, the IEF of 
      CSF from MS patients revealed only few MV-specific oligoclonal bands. In the CSF 
      samples from APME patients, intrathecal MV antibodies were not detected. 
      CONCLUSIONS: This study shows that discrimination between SSPE and MS can be 
      achieved in doubtful cases by IEF using MV-N, P and F proteins.
FAU - Pohl-Koppe, A
AU  - Pohl-Koppe A
AD  - Institut fur Virologie und Immunbiologie, Universitat Wurzburg, Versbacher 
      Strasse 7, 97078 Wurzburg, Germany.
FAU - Kaiser, R
AU  - Kaiser R
FAU - Meulen, V T
AU  - Meulen VT
FAU - Liebert, U G
AU  - Liebert UG
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Diagn Virol
JT  - Clinical and diagnostic virology
JID - 9309653
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1994/10/25 00:00 [received]
PHST- 1995/02/07 00:00 [accepted]
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 0928-0197(95)00006-T [pii]
AID - 10.1016/0928-0197(95)00006-t [doi]
PST - ppublish
SO  - Clin Diagn Virol. 1995 Aug;4(2):135-47. doi: 10.1016/0928-0197(95)00006-t.