PMID- 15569828
OWN - NLM
STAT- MEDLINE
DCOM- 20050628
LR  - 20220321
IS  - 1524-4571 (Electronic)
IS  - 0009-7330 (Linking)
VI  - 96
IP  - 1
DP  - 2005 Jan 7
TI  - Bone marrow cells differentiate in cardiac cell lineages after infarction 
      independently of cell fusion.
PG  - 127-37
AB  - Recent studies in mice have challenged the ability of bone marrow cells (BMCs) to 
      differentiate into myocytes and coronary vessels. The claim has also been made 
      that BMCs acquire a cell phenotype different from the blood lineages only by 
      fusing with resident cells. Technical problems exist in the induction of 
      myocardial infarction and the successful injection of BMCs in the mouse heart. 
      Similarly, the accurate analysis of the cell populations implicated in the 
      regeneration of the dead tissue is complex and these factors together may account 
      for the negative findings. In this study, we have implemented a simple protocol 
      that can easily be reproduced and have reevaluated whether injection of BMCs 
      restores the infarcted myocardium in mice and whether cell fusion is involved in 
      tissue reconstitution. For this purpose, c-kit-positive BMCs were obtained from 
      male transgenic mice expressing enhanced green fluorescence protein (EGFP). EGFP 
      and the Y-chromosome were used as markers of the progeny of the transplanted 
      cells in the recipient heart. By this approach, we have demonstrated that BMCs, 
      when properly administrated in the infarcted heart, efficiently differentiate 
      into myocytes and coronary vessels with no detectable differentiation into 
      hemopoietic lineages. However, BMCs have no apparent paracrine effect on the 
      growth behavior of the surviving myocardium. Within the infarct, in 10 days, 
      nearly 4.5 million biochemically and morphologically differentiated myocytes 
      together with coronary arterioles and capillary structures were generated 
      independently of cell fusion. In conclusion, BMCs adopt the cardiac cell lineages 
      and have an important therapeutic impact on ischemic heart failure.
FAU - Kajstura, Jan
AU  - Kajstura J
AD  - Cardiovascular Research Institute, Department of Medicine, New York Medical 
      College, Valhalla, NY 10595, USA. jan_kajstura@nymc.edu
FAU - Rota, Marcello
AU  - Rota M
FAU - Whang, Brian
AU  - Whang B
FAU - Cascapera, Stefano
AU  - Cascapera S
FAU - Hosoda, Toru
AU  - Hosoda T
FAU - Bearzi, Claudia
AU  - Bearzi C
FAU - Nurzynska, Daria
AU  - Nurzynska D
FAU - Kasahara, Hideko
AU  - Kasahara H
FAU - Zias, Elias
AU  - Zias E
FAU - Bonafe, Massimiliano
AU  - Bonafe M
FAU - Nadal-Ginard, Bernardo
AU  - Nadal-Ginard B
FAU - Torella, Daniele
AU  - Torella D
FAU - Nascimbene, Angelo
AU  - Nascimbene A
FAU - Quaini, Federico
AU  - Quaini F
FAU - Urbanek, Konrad
AU  - Urbanek K
FAU - Leri, Annarosa
AU  - Leri A
FAU - Anversa, Piero
AU  - Anversa P
LA  - eng
GR  - AG-023071/AG/NIA NIH HHS/United States
GR  - AG-15756/AG/NIA NIH HHS/United States
GR  - AG-17042/AG/NIA NIH HHS/United States
GR  - HL-075480/HL/NHLBI NIH HHS/United States
GR  - HL-38132/HL/NHLBI NIH HHS/United States
GR  - HL-65573/HL/NHLBI NIH HHS/United States
GR  - HL-65577/HL/NHLBI NIH HHS/United States
GR  - HL-66923/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20041129
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
CIN - Circ Res. 2005 Jan 7;96(1):6-8. PMID: 15637302
MH  - Animals
MH  - Arterioles/cytology
MH  - Artifacts
MH  - Bone Marrow Cells/*cytology
MH  - Capillaries/cytology
MH  - Cell Differentiation
MH  - Cell Fusion
MH  - *Cell Lineage
MH  - Endothelial Cells/cytology
MH  - Female
MH  - Genes, Reporter
MH  - Graft Survival
MH  - Green Fluorescent Proteins/analysis
MH  - Heart/physiology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Injections, Intralesional
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Myocardial Contraction
MH  - Myocardial Infarction/*surgery
MH  - Myocytes, Cardiac/cytology
MH  - Myocytes, Smooth Muscle/cytology
MH  - Organ Specificity
MH  - Paracrine Communication
MH  - Proto-Oncogene Proteins c-kit/analysis
MH  - Regeneration
MH  - *Stem Cell Transplantation
MH  - Ventricular Function, Left
MH  - Y Chromosome
EDAT- 2004/12/01 09:00
MHDA- 2005/06/29 09:00
CRDT- 2004/12/01 09:00
PHST- 2004/12/01 09:00 [pubmed]
PHST- 2005/06/29 09:00 [medline]
PHST- 2004/12/01 09:00 [entrez]
AID - 01.RES.0000151843.79801.60 [pii]
AID - 10.1161/01.RES.0000151843.79801.60 [doi]
PST - ppublish
SO  - Circ Res. 2005 Jan 7;96(1):127-37. doi: 10.1161/01.RES.0000151843.79801.60. Epub 
      2004 Nov 29.