PMID- 15570060
OWN - NLM
STAT- MEDLINE
DCOM- 20050113
LR  - 20230216
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 134
IP  - 12 Suppl
DP  - 2004 Dec
TI  - Transcription factor Nrf2 is essential for induction of NAD(P)H:quinone 
      oxidoreductase 1, glutathione S-transferases, and glutamate cysteine ligase by 
      broccoli seeds and isothiocyanates.
PG  - 3499S-3506S
LID - 10.1093/jn/134.12.3499S [doi]
AB  - Cruciferous vegetables contain glucosinolates that, after conversion to 
      isothiocyanates (ITC), are capable of inducing cytoprotective genes. We examined 
      whether broccoli seeds can elicit a chemoprotective response in mouse organs and 
      rodent cell lines and investigated whether this response requires nuclear 
      factor-erythroid 2 p45-related factor 2 (Nrf2). The seeds studied contained 
      glucosinolate at 40 mmol/kg, of which 59% comprised glucoiberin, 19% sinigrin, 8% 
      glucoraphanin, and 7% progoitrin. Dietary administration of broccoli seeds to 
      nrf2(+/+) and nrf2(-/-) mice produced a approximately 1.5-fold increase in 
      NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) 
      activities in stomach, small intestine, and liver of wild-type mice but not in 
      mutant mice; increased transferase activity was associated with elevated levels 
      of GSTA1/2, GSTA3, and GSTM1/2 subunits. These seeds also increased significantly 
      the level of glutamate cysteine ligase catalytic (GCLC) subunit in the stomach 
      and the small intestine of nrf2(+/+) mice but not nrf2(-/-) mice. An aqueous 
      broccoli seed extract was prepared for treatment of cultured cells that contained 
      ITC at approximately 600 mumol/L, composed of 61% 3-methylsulfinylpropyl ITC, 30% 
      sulforaphane, 4% allyl ITC, and 4% 3-butenyl ITC. This extract induced GSTA1/2, 
      GSTA3, NQO1, and GCLC between 3-fold and 10-fold in mouse Hepa-1c1c7 and rat 
      liver RL-34 cells. The broccoli seed extract affected increases in GSTA3, GSTM1, 
      and NQO1 proteins in nrf2(+/+) mouse embryonic fibroblasts but not in nrf2(-/-) 
      mouse embryonic fibroblasts. These experiments show that broccoli seeds are 
      effective at inducing antioxidant and detoxication proteins, both in vivo and ex 
      vivo, in an Nrf2-dependent manner.
FAU - McWalter, Gail K
AU  - McWalter GK
AD  - Biomedical Research Centre, Ninewells Hospital and Medical School, University of 
      Dundee, Dundee DD1 9SY, Scotland, United Kingdom.
FAU - Higgins, Larry G
AU  - Higgins LG
FAU - McLellan, Lesley I
AU  - McLellan LI
FAU - Henderson, Colin J
AU  - Henderson CJ
FAU - Song, Lijiang
AU  - Song L
FAU - Thornalley, Paul J
AU  - Thornalley PJ
FAU - Itoh, Ken
AU  - Itoh K
FAU - Yamamoto, Masayuki
AU  - Yamamoto M
FAU - Hayes, John D
AU  - Hayes JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glucosinolates)
RN  - 0 (Isothiocyanates)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Plant Extracts)
RN  - 0 (Trans-Activators)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, human)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
SB  - IM
MH  - Animals
MH  - Brassica/*chemistry
MH  - Cell Line
MH  - DNA-Binding Proteins/deficiency/genetics/*physiology
MH  - Diet
MH  - Enzyme Induction/drug effects
MH  - Gene Expression/drug effects
MH  - Glucosinolates/analysis/metabolism
MH  - Glutamate-Cysteine Ligase/*biosynthesis
MH  - Glutathione Transferase/*biosynthesis
MH  - Intestine, Small/enzymology
MH  - Isothiocyanates/administration & dosage/analysis/*pharmacology
MH  - Liver/enzymology
MH  - Mice
MH  - Mice, Knockout
MH  - Mutation
MH  - NAD(P)H Dehydrogenase (Quinone)/*biosynthesis
MH  - NF-E2-Related Factor 2
MH  - Plant Extracts/chemistry/pharmacology
MH  - Seeds/chemistry
MH  - Stomach/enzymology
MH  - Trans-Activators/deficiency/genetics/*physiology
MH  - Transfection
EDAT- 2004/12/01 09:00
MHDA- 2005/01/14 09:00
CRDT- 2004/12/01 09:00
PHST- 2004/12/01 09:00 [pubmed]
PHST- 2005/01/14 09:00 [medline]
PHST- 2004/12/01 09:00 [entrez]
AID - S0022-3166(23)03199-1 [pii]
AID - 10.1093/jn/134.12.3499S [doi]
PST - ppublish
SO  - J Nutr. 2004 Dec;134(12 Suppl):3499S-3506S. doi: 10.1093/jn/134.12.3499S.