PMID- 15570219
OWN - NLM
STAT- MEDLINE
DCOM- 20050317
LR  - 20211203
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 10
DP  - 2004 Nov 23
TI  - Cell and tissue specific expression of human Kruppel-like transcription factors 
      in human ocular surface.
PG  - 901-9
AB  - PURPOSE: The ocular surface, composed of the conjunctiva and the cornea, is 
      essential for vision. Its integrity depends on numerous molecular and cellular 
      processes such as proliferation, differentiation, apoptosis, adhesion, and 
      extracellular matrix homeostasis, whose deregulation can induce ophthalmological 
      pathologies. The Kruppel-like transcription factors (KLFs) family is made up of 
      15 C2H2 zinc-finger proteins involved in vertebrate development and able to 
      control cell proliferation, growth, and differentiation. In order to better 
      define their respective roles in the human ocular surface, we decided to 
      determine their pattern of expression in ocular tissues. We then focused on the 
      expression of KLF4 and some of its target genes to establish KLF4's biological 
      activities in human ocular surface. METHODS: Firstly, total mRNA was extracted 
      from human total cornea, conjunctiva, corneal epithelial cells (primary culture 
      and established cell line), corneal keratocytes (primary culture), corneal 
      endothelial cells (established cell line), and conjunctival epithelial cells 
      (established cell line) and submitted to RT-PCR experiments in order to determine 
      the expression patterns of the different KLFs. Secondly, KLF4 protein 
      localization was visualized by immunofluorescence assays at tissue and cell 
      levels. Finally, KLF4 target genes (endoglin, ornithine decarboxylase) mRNA 
      expression levels were determined by semi-quantitative RT-PCR, after KLF4 
      transient transfection in human corneal epithelium (HCE) cells. RESULTS: We 
      detected the presence of twelve transcripts of KLFs in the cornea (KLF2, KLF3, 
      KLF4, KLF5, KLF6, KLF7, KLF8, KLF10, KLF11, KLF12, KLF13, and KLF16) and eight in 
      the conjunctiva (KLF2, KLF3, KLF4, KLF6, KLF7, KLF10, KLF11, and KLF12). Under 
      our conditions, the transcripts encoding KLF1 and KLF9 were never detected. 
      Specific expression patterns of each KLF were also determined for the major 
      cellular components of the human cornea and conjunctiva. KLF4 immunolocalization 
      assays indicated its presence in both the cytoplasmic and nuclear compartments of 
      conjunctival and corneal cells. KLF4 transient overexpression in HCE cells down 
      regulated both endoglin and ODC mRNA levels. CONCLUSIONS: For the first time, we 
      established the presence of a KLF network in the human ocular surface and 
      illustrated the conservation of KLF4's biological properties in a corneal derived 
      epithelial cell line.
FAU - Chiambaretta, Frederic
AU  - Chiambaretta F
AD  - Department of Ophthalmology, University Hospital, CHU, Clermont-Ferrand, France.
FAU - De Graeve, Fabienne
AU  - De Graeve F
FAU - Turet, Gilles
AU  - Turet G
FAU - Marceau, Geoffroy
AU  - Marceau G
FAU - Gain, Philippe
AU  - Gain P
FAU - Dastugue, Bernard
AU  - Dastugue B
FAU - Rigal, Daniele
AU  - Rigal D
FAU - Sapin, Vincent
AU  - Sapin V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041123
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Antigens, CD)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ENG protein, human)
RN  - 0 (Endoglin)
RN  - 0 (KLF4 protein, human)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
SB  - IM
MH  - Antigens, CD
MH  - Cell Line
MH  - Conjunctiva/*metabolism
MH  - DNA-Binding Proteins/*genetics/*metabolism
MH  - Endoglin
MH  - Endothelium, Corneal/metabolism
MH  - Epithelium/metabolism
MH  - Epithelium, Corneal/*metabolism
MH  - Fibroblasts/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Gene Expression
MH  - Humans
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors
MH  - Ornithine Decarboxylase/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Cell Surface
MH  - Repressor Proteins/*genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/*genetics/*metabolism
MH  - Transfection
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
MH  - Zinc Fingers/*genetics
EDAT- 2004/12/01 09:00
MHDA- 2005/03/18 09:00
CRDT- 2004/12/01 09:00
PHST- 2004/12/01 09:00 [pubmed]
PHST- 2005/03/18 09:00 [medline]
PHST- 2004/12/01 09:00 [entrez]
AID - v10/a108 [pii]
PST - epublish
SO  - Mol Vis. 2004 Nov 23;10:901-9.