PMID- 15571977
OWN - NLM
STAT- MEDLINE
DCOM- 20050307
LR  - 20131121
IS  - 0969-9961 (Print)
IS  - 0969-9961 (Linking)
VI  - 17
IP  - 3
DP  - 2004 Dec
TI  - Resistance of neurofilaments to degradation, and lack of neuronal death and mossy 
      fiber sprouting after kainic acid-induced status epilepticus in the developing 
      rat hippocampus.
PG  - 415-26
AB  - Neurofilament (NF) proteins, the major constituent of intermediate filaments in 
      neurons, have an important role in cellular stability and plasticity. We have now 
      studied the short-term (hours) and long-term (up to 1 week) effects of kainic 
      acid (KA)-induced status epilepticus (SE) on the reactivity of NF proteins, and 
      mossy fiber (MF) sprouting and neuronal death up to 4 weeks in 9-day-old rats. In 
      Western blotting, the expression of the phosphorylation-independent epitopes of 
      NF-L, NF-M, and NF-H rapidly but transiently increased after the treatment, 
      whereas the phosphorylated NF-M remained elevated for 7 days. However, the 
      treatment did not change the immunoreactivity of NF proteins, and no neuronal 
      death or mossy fiber sprouting was detected at any time point. Our findings 
      indicate seizure-induced reactivity of NF proteins but their resistance to 
      degradation, which could be of importance in neuronal survival and may also 
      prevent MF sprouting in the developing hippocampus.
FAU - Lopez-Picon, Francisco
AU  - Lopez-Picon F
AD  - Department of Pharmacology and Clinical Pharmacology, FIN-20520 Turku, Finland.
FAU - Puustinen, Niina
AU  - Puustinen N
FAU - Kukko-Lukjanov, Tiina-Kaisa
AU  - Kukko-Lukjanov TK
FAU - Holopainen, Irma E
AU  - Holopainen IE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Neurofilament Proteins)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Death/drug effects
MH  - Disease Models, Animal
MH  - Hippocampus/drug effects/*pathology
MH  - Intermediate Filaments/*pathology/ultrastructure
MH  - Kainic Acid/*toxicity
MH  - Neurofilament Proteins/drug effects/metabolism
MH  - Neurons/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Status Epilepticus/chemically induced/*pathology
MH  - Time Factors
EDAT- 2004/12/02 09:00
MHDA- 2005/03/08 09:00
CRDT- 2004/12/02 09:00
PHST- 2003/12/09 00:00 [received]
PHST- 2004/08/05 00:00 [revised]
PHST- 2004/08/18 00:00 [accepted]
PHST- 2004/12/02 09:00 [pubmed]
PHST- 2005/03/08 09:00 [medline]
PHST- 2004/12/02 09:00 [entrez]
AID - S0969-9961(04)00187-1 [pii]
AID - 10.1016/j.nbd.2004.08.004 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2004 Dec;17(3):415-26. doi: 10.1016/j.nbd.2004.08.004.