PMID- 15573249
OWN - NLM
STAT- MEDLINE
DCOM- 20050208
LR  - 20181113
IS  - 0364-2313 (Print)
IS  - 0364-2313 (Linking)
VI  - 28
IP  - 10
DP  - 2004 Oct
TI  - Altered serum transforming growth factor-beta1 and monocyte chemoattractant 
      protein-1 levels in obstructive jaundice.
PG  - 967-70
AB  - Impaired immune function has long been documented in patients with obstructive 
      jaundice, and those with jaundice due to extrahepatic biliary obstruction still 
      experience a high rate of postoperative complications and death. Transforming 
      growth factor-beta1 (TGFbeta1) appears to be an important regulator of both 
      normal and pathologic conditions in the liver. Monocyte chemoattractant protein-1 
      (MCP-1) is an important mediator of monocyte recruitment to inflammatory sites. 
      We hypothesize that obstructive jaundice may alter serum TGFbeta1 and MCP-1 
      expressions in the rat and that oral bile acid or glutamine (or both) can restore 
      the altered serum TGFbeta1 and MCP-1 expression in rats with obstructive 
      jaundice. Male Sprague-Dawley rats weighing 250 to 300 g were randomized to four 
      groups (n = 10 in each group). Group 1 underwent a sham operation with oral 
      normal saline administration. Group 2 underwent common bile duct ligation (CBDL) 
      with oral normal saline administration. Group 3 underwent CBDL with oral bile 
      acid replacement. Group 4 underwent CBDL with oral glutamine administration. 
      Animals were sacrificed after 3 days (n = 5) and 7 days (n = 5), and blood 
      samples were collected. Serum was obtained after centrifugation for measurement 
      of TGFbeta1 and MCP-1 levels by an enzyme-linked immunosorbent assay. The serum 
      TGFbeta1 level was significantly elevated (p = 0.006) 3 days after CBDL. Oral 
      glutamine administration prevented this elevation, but oral bile acid replacement 
      did not. The serum MCP-1 level showed similar changes. After 3 days of 
      obstructive jaundice, the TGFbeta1 and MCP-1 levels were altered in the rat. Oral 
      glutamine administration, not oral bile acid replacement, was able to prevent 
      these alterations.
FAU - Sheen-Chen, Shyr-Ming
AU  - Sheen-Chen SM
AD  - Department of Surgery, College of Medicine, Chang Gung University, Chang Gung 
      Memorial Hospital, Kaohsiung, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien, 
      Taiwan. smsheen@yahoo.com
FAU - Eng, Hock-Liew
AU  - Eng HL
FAU - Hung, Kuo-Sheng
AU  - Hung KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040929
PL  - United States
TA  - World J Surg
JT  - World journal of surgery
JID - 7704052
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*blood
MH  - Jaundice, Obstructive/*blood
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta/*blood
MH  - Transforming Growth Factor beta1
EDAT- 2004/12/02 09:00
MHDA- 2005/02/09 09:00
CRDT- 2004/12/02 09:00
PHST- 2004/12/02 09:00 [pubmed]
PHST- 2005/02/09 09:00 [medline]
PHST- 2004/12/02 09:00 [entrez]
AID - 10.1007/s00268-004-7354-z [doi]
PST - ppublish
SO  - World J Surg. 2004 Oct;28(10):967-70. doi: 10.1007/s00268-004-7354-z. Epub 2004 
      Sep 29.