PMID- 15577316
OWN - NLM
STAT- MEDLINE
DCOM- 20050816
LR  - 20191026
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 14
IP  - 6
DP  - 2004 Dec
TI  - Differential expression of melanoma-associated antigens and molecules involved in 
      antigen processing and presentation in three cell lines established from a single 
      patient.
PG  - 463-71
AB  - Tumour cells are able to evade the immune system by using several 'escape 
      mechanisms'. Downregulation of molecules involved in the processing and 
      presentation of self-antigens has been reported. However, these adaptations have 
      not been compared in metastases in different anatomical locations but derived 
      from a single patient. We investigated three melanoma cell lines--MJT1 from the 
      parietal lobe of the brain, MJT3 from the cerebellum and MJT5 from the left side 
      of the neck--established from biopsies excised from a 45 year old female patient. 
      Although human leukocyte antigen (HLA) class I was detected in all three cell 
      lines by flow cytometry using an anti-HLA monomorphic antibody, further 
      serological analysis demonstrated HLA B38 loss in all three cell lines, HLA B7 
      downregulation in MJT5 (skin metastases) and B7 loss in MJT3 and MJT1 (brain 
      metastases) compared with the HLA type of the patient's normal autologous 
      lymphocytes. Interferon-gamma (IFNgamma) treatment increased the expression of 
      HLA class I and transporters associated with antigen processing 1 (TAP1) in all 
      three cell lines. De novo HLA class II molecule expression was observed after 
      IFNgamma treatment in MJT3 and MJT5. Western blot and reverse 
      transcription-polymerase chain reaction results revealed heterogeneity of 
      melanoma-associated antigen (MAA) expression in the cell lines: MJT3 cells 
      expressed higher levels of MAAs than the other two cell lines. In conclusion, 
      this study has demonstrated that three metastatic lesions from a single patient 
      can have differential expression of molecules involved in antigen processing 
      (TAP1) and presentation (HLA I and II), but that expression of these molecules is 
      modulated by IFNgamma to a similar degree in all cell lines. In contrast, the 
      downregulation of expression of specific MAAs between the three cell lines was 
      unaffected by the addition of IFNgamma.
FAU - Kovalcsik, Edit
AU  - Kovalcsik E
AD  - Department of Oncology, St. George's Hospital Medical School, London, UK.
FAU - John, Justin
AU  - John J
FAU - Turner, Matthew
AU  - Turner M
FAU - Birchall, Lindsay
AU  - Birchall L
FAU - Sage, Deborah
AU  - Sage D
FAU - Whittle, Robert
AU  - Whittle R
FAU - Dalgleish, Angus
AU  - Dalgleish A
FAU - Pandha, Hardev
AU  - Pandha H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B38 Antigen)
RN  - 0 (HLA-B7 Antigen)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Melanoma-Specific Antigens)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (TAP1 protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - *Antigen Presentation
MH  - Antigens, Neoplasm
MH  - Antineoplastic Agents/pharmacology
MH  - Brain Neoplasms/immunology/*metabolism/secondary
MH  - Female
MH  - HLA-B Antigens/genetics/metabolism
MH  - HLA-B38 Antigen
MH  - HLA-B7 Antigen/genetics/metabolism
MH  - Histocompatibility Antigens Class I/*genetics/metabolism
MH  - Histocompatibility Antigens Class II/*genetics/metabolism
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Lymphocytes/metabolism
MH  - Melanoma/immunology/*metabolism/secondary
MH  - Melanoma-Specific Antigens
MH  - Middle Aged
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Skin Neoplasms/immunology/*metabolism/secondary
MH  - Tumor Cells, Cultured
EDAT- 2004/12/04 09:00
MHDA- 2005/08/17 09:00
CRDT- 2004/12/04 09:00
PHST- 2004/12/04 09:00 [pubmed]
PHST- 2005/08/17 09:00 [medline]
PHST- 2004/12/04 09:00 [entrez]
AID - 00008390-200412000-00005 [pii]
AID - 10.1097/00008390-200412000-00005 [doi]
PST - ppublish
SO  - Melanoma Res. 2004 Dec;14(6):463-71. doi: 10.1097/00008390-200412000-00005.