PMID- 15578074
OWN - NLM
STAT- MEDLINE
DCOM- 20050624
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 18
IP  - 5
DP  - 2005 May
TI  - Expression profile and molecular genetic regulation of cyclin D1 expression in 
      epithelioid sarcoma.
PG  - 705-9
AB  - Epithelioid sarcoma is a distinctive, aggressive soft tissue tumor typically 
      presenting as a subcutaneous or deep dermal mass in the distal extremities of 
      young adults. Molecular genetic data of well-characterized cases of epithelioid 
      sarcoma are sparse. A recent cytogenetic study of epithelioid sarcoma by 
      conventional metaphase comparative genomic hybridization reported recurrent gains 
      at chromosome 11q13, a region containing many genes, including the cyclin D1 
      gene. Cyclin D1 is a positive cell cycle regulator that is overexpressed in a 
      variety of neoplasms, including mantle cell lymphoma and breast carcinoma. The 
      objective of this study was to examine cyclin D1 expression in epithelioid 
      sarcoma. Of 24 cases evaluated, 23 (96%) displayed cyclin D1 nuclear expression 
      using immunohistochemical evaluation. Eight cases, which expressed cyclin D1 by 
      immunohistochemistry, were evaluated by fluorescence in situ hybridization (FISH) 
      and RNA in situ hybridization (RISH) for amplification of the cyclin D1 gene and 
      messenger RNA (mRNA) expression, respectively. Seven of eight cases showed a 
      typical eusomic state. One case showed pseudoamplification due to 
      aneusomy/polysomy. There was no evidence of cyclin D1 gene amplification or 
      messenger RNA overexpression detected by FISH or RNA in situ hybridization 
      analyses, respectively. Our data clearly demonstrate that cyclin D1 protein is 
      upregulated in epithelioid sarcoma, suggesting a role for this cell cycle 
      regulator in the pathogenesis of epithelioid sarcoma. The high level of cyclin D1 
      protein expression in epithelioid sarcoma appears to be regulated by 
      translational and/or post-translational mechanisms.
FAU - Lin, Lin
AU  - Lin L
AD  - Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
FAU - Hicks, David
AU  - Hicks D
FAU - Xu, Bo
AU  - Xu B
FAU - Sigel, Jessica E
AU  - Sigel JE
FAU - Bergfeld, Wilma F
AU  - Bergfeld WF
FAU - Montgomery, Elizabeth
AU  - Montgomery E
FAU - Fisher, Cyril
AU  - Fisher C
FAU - Hartke, Marybeth
AU  - Hartke M
FAU - Tubbs, Raymond
AU  - Tubbs R
FAU - Goldblum, John R
AU  - Goldblum JR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (RNA, Messenger)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Cyclin D1/analysis/*genetics
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Messenger/genetics/metabolism
MH  - Sarcoma/genetics/metabolism/*pathology
MH  - U937 Cells
EDAT- 2004/12/04 09:00
MHDA- 2005/06/25 09:00
CRDT- 2004/12/04 09:00
PHST- 2004/12/04 09:00 [pubmed]
PHST- 2005/06/25 09:00 [medline]
PHST- 2004/12/04 09:00 [entrez]
AID - S0893-3952(22)04645-2 [pii]
AID - 10.1038/modpathol.3800349 [doi]
PST - ppublish
SO  - Mod Pathol. 2005 May;18(5):705-9. doi: 10.1038/modpathol.3800349.