PMID- 15578270
OWN - NLM
STAT- MEDLINE
DCOM- 20050408
LR  - 20220310
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 318
IP  - 3
DP  - 2004 Dec
TI  - Increased noise sensitivity and altered inner ear MENA distribution in VASP-/- 
      mice.
PG  - 493-502
AB  - Vasodilator-stimulated phosphoprotein (VASP) and mammalian-enabled protein (MENA) 
      share similar cellular localisation and functions (signal transduction pathways, 
      regulation of actin cytoskeleton dynamics). Functional substitution and 
      compensation among Ena/VASP proteins have been proposed as the reason for the 
      absence of major morphological and functional deficits in VASP-/- mice. The aim 
      of this study was to investigate VASP expression in the mouse cochlea, to analyse 
      cochlear function in VASP-/- mice compared with wildtype mice, and to analyse 
      cochlear MENA distribution taking into account that MENA protein might compensate 
      VASP loss in the cochlea of VASP-/- mice. We confirmed specific VASP expression 
      in the pillar cells of the mice organ of Corti as previously reported for rat 
      cochlea. By analysing the hearing function in VASP-/- mice, we found no 
      differences in auditory brainstem responses and distortion product otoacoustic 
      emissions from those of wildtype mice but evidence for an increased noise 
      sensitivity at lower frequencies. When MENA protein levels in cochlea tissue were 
      tested in mutant and wildtype mice by Western blot analysis, no significant 
      differences were found, as was also seen with regard to MENA mRNA levels in 
      laser-microdissected single pillar cells. Most surprisingly, however, MENA 
      protein was absent in pillar cells of VASP-/- mice, whereas it was detected in 
      other cochlear cells. The finding of a cell-specific, and not organ-specific, 
      redundancy of MENA protein expression noted for the first time in VASP-/- mice is 
      proposed as the reason for the observed distinct cochlear phenotype.
FAU - Schick, Bernhard
AU  - Schick B
AD  - Department of Otolaryngology, University Erlangen-Nurnberg, Waldstrasse 1, 91054, 
      Erlangen, Germany. bernhard.schick@hno.imed.uni-erlangen.de
FAU - Praetorius, Mark
AU  - Praetorius M
FAU - Eigenthaler, Martin
AU  - Eigenthaler M
FAU - Jung, Volker
AU  - Jung V
FAU - Muller, Marcus
AU  - Muller M
FAU - Walter, Ulrich
AU  - Walter U
FAU - Knipper, Marlies
AU  - Knipper M
LA  - eng
PT  - Journal Article
DEP - 20041002
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Enah protein, mouse)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (vasodilator-stimulated phosphoprotein)
SB  - IM
MH  - Animals
MH  - Auditory Threshold/*physiology
MH  - Blotting, Western
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Cytoskeletal Proteins/genetics/*metabolism
MH  - Evoked Potentials, Auditory, Brain Stem/physiology
MH  - Gene Expression
MH  - Hearing Loss, Noise-Induced/physiopathology
MH  - In Situ Hybridization
MH  - Labyrinth Supporting Cells/*metabolism/pathology/ultrastructure
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microfilament Proteins
MH  - Otoacoustic Emissions, Spontaneous/physiology
MH  - Phosphoproteins/genetics/*metabolism
MH  - RNA, Messenger/metabolism
EDAT- 2004/12/04 09:00
MHDA- 2005/04/09 09:00
CRDT- 2004/12/04 09:00
PHST- 2004/01/02 00:00 [received]
PHST- 2004/06/26 00:00 [accepted]
PHST- 2004/12/04 09:00 [pubmed]
PHST- 2005/04/09 09:00 [medline]
PHST- 2004/12/04 09:00 [entrez]
AID - 10.1007/s00441-004-0964-9 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2004 Dec;318(3):493-502. doi: 10.1007/s00441-004-0964-9. Epub 
      2004 Oct 2.