PMID- 15578884
OWN - NLM
STAT- MEDLINE
DCOM- 20050520
LR  - 20210527
IS  - 1543-2165 (Electronic)
IS  - 0003-9985 (Linking)
VI  - 128
IP  - 12
DP  - 2004 Dec
TI  - The value of fluorescence in situ hybridization and polymerase chain reaction in 
      the diagnosis of B-cell non-Hodgkin lymphoma by fine-needle aspiration.
PG  - 1395-403
AB  - CONTEXT: Molecular genetic analyses have been predicted to improve the diagnostic 
      accuracy of fine-needle aspiration of B-cell non-Hodgkin lymphoma. OBJECTIVE: To 
      determine the value of routine molecular genetic assays, polymerase chain 
      reaction (PCR) and fluorescence in situ hybridization (FISH), in the diagnosis of 
      B-cell non-Hodgkin lymphoma by fine-needle aspiration (FNA). DESIGN: A 
      multiparametric method, including cytology, flow cytometry, PCR, and FISH, was 
      prospectively evaluated in the diagnosis of B-cell non-Hodgkin lymphoma by FNA. 
      Aspirates from 30 consecutive patients with suspected hematolymphoid malignancies 
      were collected. All aspirates were triaged through a uniform program including 
      cell-size analysis, B- and T-cell clonality studies, flow cytometric 
      immunophenotyping, and bcl-1 and bcl-2 gene rearrangements by PCR and FISH. After 
      completion of FNA evaluations, FNA results were compared with diagnoses from 
      prior or subsequent surgical biopsies. RESULTS: Monoclonal B-cell populations 
      were detected in 18 of 20 B-cell non-Hodgkin lymphomas by flow cytometry and PCR. 
      bcl-1 gene rearrangement was detected in 2 of 2 cases of mantle cell lymphoma. 
      bcl-2 rearrangement was detected in 5 cases including 4 of 4 low-grade follicular 
      lymphomas and 1 transformed follicular lymphoma. By incorporating the results of 
      molecular genetic and ancillary diagnostics, a definitive classification was 
      reached in 12 cases of B-cell non-Hodgkin lymphoma by FNA, including all cases of 
      low-grade follicular lymphoma (4/4) and mantle cell lymphoma (2/2) and 
      approximately 50% of small lymphocytic lymphoma (2/4) and large B-cell lymphoma 
      (4/8). Ten of the 12 cases with a final classification reached by FNA had either 
      prior or follow-up surgical biopsies, and all 10 cases showed agreement between 
      the diagnoses rendered on FNA and surgical biopsies. CONCLUSIONS: With proper 
      handling and management of specimens, FNA can routinely provide samples adequate 
      for molecular genetic studies, in addition to cytomorphology and flow cytometry, 
      making it possible to consistently render accurate and definitive diagnoses in a 
      subset of B-cell non-Hodgkin lymphomas. By incorporating FISH and PCR methods, 
      FNA may assume an expanded role for the primary diagnosis of B-cell non-Hodgkin 
      lymphoma.
FAU - Safley, Anne M
AU  - Safley AM
AD  - Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Buckley, Patrick J
AU  - Buckley PJ
FAU - Creager, Andrew J
AU  - Creager AJ
FAU - Dash, Rajesh C
AU  - Dash RC
FAU - Dodd, Leslie G
AU  - Dodd LG
FAU - Goodman, Barbara K
AU  - Goodman BK
FAU - Jones, Claudia K
AU  - Jones CK
FAU - Lagoo, Anand S
AU  - Lagoo AS
FAU - Stenzel, Timothy T
AU  - Stenzel TT
FAU - Wang, Weihua
AU  - Wang W
FAU - Xie, Bill
AU  - Xie B
FAU - Gong, Jerald Z
AU  - Gong JZ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Pathol Lab Med
JT  - Archives of pathology & laboratory medicine
JID - 7607091
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Fine-Needle
MH  - Cytodiagnosis/trends
MH  - Female
MH  - Flow Cytometry/trends
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods/*trends
MH  - Lymphoma, B-Cell/*diagnosis
MH  - Lymphoma, Non-Hodgkin/*diagnosis
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/trends
MH  - Polymerase Chain Reaction/methods/*trends
MH  - Prospective Studies
EDAT- 2004/12/08 09:00
MHDA- 2005/05/21 09:00
CRDT- 2004/12/08 09:00
PHST- 2004/12/08 09:00 [pubmed]
PHST- 2005/05/21 09:00 [medline]
PHST- 2004/12/08 09:00 [entrez]
AID - OA4109 [pii]
AID - 10.5858/2004-128-1395-TVOFIS [doi]
PST - ppublish
SO  - Arch Pathol Lab Med. 2004 Dec;128(12):1395-403. doi: 
      10.5858/2004-128-1395-TVOFIS.