PMID- 15579413
OWN - NLM
STAT- MEDLINE
DCOM- 20050302
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Linking)
VI  - 112
IP  - 17
DP  - 2004 Dec
TI  - The sources of inflammatory mediators in the lung after silica exposure.
PG  - 1679-86
AB  - The expression of 10 genes implicated in regulation of the inflammatory processes 
      in the lung was studied after exposure of alveolar macrophages (AMs) to silica in 
      vitro or in vivo. Exposure of AMs to silica in vitro up-regulated the messenger 
      RNA (mRNA) levels of three genes [interleukin-6 (IL-6), monocyte chemoattractant 
      protein-1 (MCP-1), and macrophage inflammatory protein-2 (MIP-2)] without a 
      concomitant increase in the protein levels. AMs isolated after intratracheal 
      instillation of silica up-regulated mRNA levels of four additional genes 
      [granulocyte/macrophage-colony stimulating factor (GM-CSF), IL-1beta, IL-10, and 
      inducible nitric oxide synthase]. IL-6, MCP-1, and MIP-2 protein levels were 
      elevated in bronchoalveolar lavage fluid. Fibroblasts under basal culture 
      conditions express much higher levels of IL-6 and GM-CSF compared with AMs. 
      Coculture of AMs and alveolar type II cells, or coculture of AMs and lung 
      fibroblasts, in contact cultures or Transwell chambers, revealed no synergistic 
      effect. Therefore, such interaction does not explain the effects seen in vivo. 
      Identification of the intercellular communication in vivo is still unresolved. 
      However, fibroblasts appear to be an important source of inflammatory mediators 
      in the lung.
FAU - Rao, K Murali Krishna
AU  - Rao KM
AD  - Pathology and Physiology Research Branch, Health Effects Laboratory Division, 
      National Institute for Occupational Safety and Health, Morgantown, West Virginia, 
      USA. mir8@cdc.gov
FAU - Porter, Dale W
AU  - Porter DW
FAU - Meighan, Terence
AU  - Meighan T
FAU - Castranova, Vince
AU  - Castranova V
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Cytokines/*biosynthesis
MH  - Fibroblasts/physiology
MH  - Gene Expression Regulation/*drug effects
MH  - Inflammation/*physiopathology
MH  - Lung/*immunology/*pathology
MH  - Macrophages, Alveolar
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Silicon Dioxide/*toxicity
MH  - Up-Regulation
PMC - PMC1253659
EDAT- 2004/12/08 09:00
MHDA- 2005/03/03 09:00
PMCR- 2004/12/01
CRDT- 2004/12/08 09:00
PHST- 2004/12/08 09:00 [pubmed]
PHST- 2005/03/03 09:00 [medline]
PHST- 2004/12/08 09:00 [entrez]
PHST- 2004/12/01 00:00 [pmc-release]
AID - ehp0112-001679 [pii]
AID - 10.1289/ehp.7295 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2004 Dec;112(17):1679-86. doi: 10.1289/ehp.7295.