PMID- 15579464
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 280
IP  - 7
DP  - 2005 Feb 18
TI  - Hemoglobin receptor in Leishmania is a hexokinase located in the flagellar 
      pocket.
PG  - 5884-91
AB  - Hb endocytosis in Leishmania is mediated through a 46-kDa protein located in the 
      flagellar pocket. To understand the nature of the Hb receptor (HbR), we have 
      purified the 46-kDa protein to homogeneity from Leishmania promastigote membrane. 
      Purified HbR specifically binds Hb. The gene for HbR was cloned, and sequence 
      analysis of the full-length HbR gene indicates the presence of hexokinase (HK) 
      signature sequences, ATP-binding domain, and PTS-II motif. Four lines of evidence 
      indicate that HbR in Leishmania is a hexokinase: 1) the recombinant HbR binds Hb, 
      and the Hb-binding domain resides in the N terminus of the protein; 2) 
      recombinant proteins and cell lysate prepared from HbR-overexpressing Leishmania 
      promastigotes show enhanced HK activity in comparison with untransfected cells; 
      3) immunolocalization studies using antibodies against the N-terminal fragment 
      (Ld-HbR-DeltaC) of Ld-HbR indicate that this protein is located in the flagellar 
      pocket of Leishmania; and 4) binding and uptake of (125)I-Hb by Leishmania is 
      significantly inhibited by anti-Ld-HbR-DeltaC antibody and Ld-HbR-DeltaC, 
      respectively. Taken together, these results indicate that HK present in the 
      flagellar pocket of Leishmania is involved in Hb endocytosis.
FAU - Krishnamurthy, Ganga
AU  - Krishnamurthy G
AD  - National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India.
FAU - Vikram, Rajagopal
AU  - Vikram R
FAU - Singh, Sudha B
AU  - Singh SB
FAU - Patel, Nitin
AU  - Patel N
FAU - Agarwal, Shruti
AU  - Agarwal S
FAU - Mukhopadhyay, Gauranga
AU  - Mukhopadhyay G
FAU - Basu, Sandip K
AU  - Basu SK
FAU - Mukhopadhyay, Amitabha
AU  - Mukhopadhyay A
LA  - eng
SI  - GENBANK/AY659996
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041203
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Hemoglobins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - Flagella/*physiology
MH  - Hemoglobins/*metabolism
MH  - Hexokinase/chemistry/genetics/isolation & purification/*metabolism
MH  - Kinetics
MH  - Leishmania donovani/enzymology/genetics/growth & development/*metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Protozoan Proteins/chemistry/genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism
MH  - Substrate Specificity
EDAT- 2004/12/08 09:00
MHDA- 2005/04/19 09:00
CRDT- 2004/12/08 09:00
PHST- 2004/12/08 09:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2004/12/08 09:00 [entrez]
AID - S0021-9258(19)63095-4 [pii]
AID - 10.1074/jbc.M411845200 [doi]
PST - ppublish
SO  - J Biol Chem. 2005 Feb 18;280(7):5884-91. doi: 10.1074/jbc.M411845200. Epub 2004 
      Dec 3.