PMID- 15580303
OWN - NLM
STAT- MEDLINE
DCOM- 20050215
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 24
IP  - 4
DP  - 2005 Jan 20
TI  - Parity-induced mouse mammary epithelial cells are pluripotent, self-renewing and 
      sensitive to TGF-beta1 expression.
PG  - 552-60
AB  - A parity-induced mammary population, marked by beta-galactosidase expression 
      conditionally activated through cre-lox recombinase originates in 
      WAP-Cre/Rosa-lox-STOP-lox-LacZ (WAP-Cre/Rosa-LacZ) female mice during pregnancy, 
      lactation and involution. During subsequent pregnancies, these parity-induced 
      mammary epithelial cells (PI-MEC) proliferated to produce new secretory acini 
      composed of secretory luminal cells and myoepithelium. In serial transplantation 
      assays, PI-MEC were able to self-renew over several transplant generations and to 
      contribute significantly to the resulting mammary outgrowths. In limiting 
      dilution transplantation, they proliferated to produce both luminal and 
      myoepithelial cells, comprised both lobule-limited and duct-limited epithelial 
      outgrowths, and differentiated into all the cellular subtypes recognized in 
      murine mammary epithelium. TGF-beta1 expression from the whey acidic protein 
      promoter (WAP) in triply transgenic females did not prevent the appearance of 
      PI-MEC after pregnancy despite the absence of full lactation or their ability to 
      proliferate and produce progeny with diverse cellular fates in situ upon 
      subsequent pregnancies. However, in transplants from triple transgenic parous 
      females, the WAP-TGF-beta1-positive PI-MEC did not contribute to the newly 
      recapitulated mammary outgrowths, suggesting that they were incapable of 
      expansive cellular proliferation (self-renewal). This result is consistent with 
      our earlier publication that WAP-TGF-beta1 expression in mammary epithelium 
      induces premature stem cell senescence in mammary transplants and decreases 
      mammary cancer risk in mouse mammary tumor virus (MMTV)-infected females even 
      after multiple pregnancies.
FAU - Boulanger, Corinne A
AU  - Boulanger CA
AD  - Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, 
      Bethesda, MD 20892, USA.
FAU - Wagner, Kay-Uwe
AU  - Wagner KU
FAU - Smith, Gilbert H
AU  - Smith GH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Milk Proteins)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Viral Proteins)
RN  - 0 (whey acidic proteins)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Animals
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Cell Transplantation
MH  - Cells, Cultured
MH  - Epithelial Cells/*cytology/metabolism
MH  - Female
MH  - Integrases/metabolism
MH  - Lac Operon/genetics
MH  - Male
MH  - Mammary Glands, Animal/*cytology/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Milk Proteins/genetics/metabolism
MH  - Parity/*physiology
MH  - Pluripotent Stem Cells/*cytology/metabolism
MH  - Transforming Growth Factor beta/*metabolism
MH  - Transforming Growth Factor beta1
MH  - Viral Proteins/metabolism
EDAT- 2004/12/08 09:00
MHDA- 2005/02/16 09:00
CRDT- 2004/12/08 09:00
PHST- 2004/12/08 09:00 [pubmed]
PHST- 2005/02/16 09:00 [medline]
PHST- 2004/12/08 09:00 [entrez]
AID - 1208185 [pii]
AID - 10.1038/sj.onc.1208185 [doi]
PST - ppublish
SO  - Oncogene. 2005 Jan 20;24(4):552-60. doi: 10.1038/sj.onc.1208185.