PMID- 15581220
OWN - NLM
STAT- MEDLINE
DCOM- 20050418
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 68
IP  - 4
DP  - 2004 Oct
TI  - Evaluation of the presence of porcine reproductive and respiratory syndrome virus 
      in pig meat and experimental transmission following oral exposure.
PG  - 259-66
AB  - A study was performed to evaluate the presence of porcine reproductive and 
      respiratory syndrome virus (PRRSV) in pig meat collected at slaughterhouses and 
      its potential transmission to pigs via pig meat. A total of 1039 blood samples 
      were collected from pigs upon their arrival at the abattoir. The following day, 
      meat samples (n = 1027) were collected from the carcasses of these same pigs. 
      Samples originated from 2 Canadian slaughterhouses, 1 situated in the province of 
      Quebec and the other situated in the province of Manitoba. Serum samples were 
      tested for antibodies to PRRSV and both serum and meat samples were also tested 
      for PRRSV nucleic acid by polymerase chain reaction (PCR). Seropositivity to 
      PRRSV for all serum samples was 74.3%. Furthermore 45 (4.3%) of the total serum 
      samples and 19 (1.9%) of the 1027 meat samples were positive for PRRSV by PCR. 
      Sequence analysis of open reading frame (ORF) 5 performed on 15 of the 19 PRRSV 
      strains identified in pig meat indicated that 9 were field strains and 6 were 
      vaccine-like (98% to 99.7% nucleotide homology with the Ingelvac RespPRRS/Repro 
      vaccine). One of these 6 strains presented an intermediate 2-6-2 restriction 
      fragment length polymorphism (RFLP) cut pattern and the others showed the 
      characteristic 2-5-2 RFLP pattern of the vaccine strain. All strains sequenced 
      were determined to be North American strains. In only 1 of the 19 PRRSV-positive 
      meat samples could PRRSV be isolated. To test the potential infectivity of meat 
      samples containing residual PRRSV, 11 of the PCR-positive meat samples (weighing 
      1.05 to 1.8 kg) were each used in feeding experiments of 2 PRRSV 
      antibody-negative specific pathogen-free pigs of 9 wk of age. Samples were cut 
      into several pieces and fed to each pair of pigs on 2 consecutive days. Each pig 
      pair was housed in a separate cubicle and serum samples were collected at -7, 0, 
      7, 14, and 20 to 21 days post exposure. Seven pig pairs were found to be infected 
      by PRRSV following ingestion of meat samples, including meat samples containing 
      vaccine-like virus, as judged by the demonstration of PRRSV antibodies and/or 
      PRRSV nucleic acid in the serum. In summary, the present study indicated that low 
      residual quantities of PRRSV may be found in a small percentage of pig meat 
      collected at slaugtherhouses. Furthermore, when this meat was fed raw to pigs in 
      the experimental setting designed, pigs could be infected by PRRSV.
FAU - Magar, Ronald
AU  - Magar R
AD  - Agence canadienne d'inspection des aliments, Laboratoire de Saint-Hyacinthe, 
      Saint-Hyacinthe, Quebec J2S 8E3. magarr@inspection.gc.ca
FAU - Larochelle, Renee
AU  - Larochelle R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Abattoirs
MH  - Animal Feed
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - *Food Contamination
MH  - Meat/*virology
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Swine
PMC - PMC1111356
EDAT- 2004/12/08 09:00
MHDA- 2005/04/19 09:00
PMCR- 2005/01/01
CRDT- 2004/12/08 09:00
PHST- 2004/12/08 09:00 [pubmed]
PHST- 2005/04/19 09:00 [medline]
PHST- 2004/12/08 09:00 [entrez]
PHST- 2005/01/01 00:00 [pmc-release]
AID - pg259 [pii]
PST - ppublish
SO  - Can J Vet Res. 2004 Oct;68(4):259-66.