PMID- 15583829
OWN - NLM
STAT- MEDLINE
DCOM- 20050516
LR  - 20061115
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 15
IP  - 1
DP  - 2005 Jan
TI  - Hepatocyte growth factor/scatter factor induces VEGF in human external auditory 
      canal cholesteatoma cell culture.
PG  - 67-71
AB  - Human external auditory canal cholesteatoma (EACC) is not often seen in 
      otolaryngology. Some authors have noted circulatory disorders of the local blood 
      vessels as the etiologic factor for establishing EACC. Diminished oxygen supply 
      results in the attempt to establish angiogenesis. Hepatocyte growth 
      factor/scatter factor (HGF/SF) and vascular endothelial growth factor (VEGF) are 
      the most important angiogenic factors in this process. In a recent study we 
      described strong expression of VEGF and HGF in EACC. All EACC and normal AMS cell 
      cultures were obtained from 5 patients undergoing surgery and used at passage 3. 
      After 16 to 72 h of incubation with 20 ng/ml HGF/SF, the expression of the VEGF 
      protein in the supernants of the HGF/SF-treated and untreated culture was 
      analyzed. EACC-culture cells showed a stronger baseline expression of VEGF. After 
      72 h of incubation with 20 ng/ml HGF/SF of HGF/SF, the expression of VEGF in 
      normal keratinocytes was 173.4 pg/ml. The expression level of VEGF in the EACC 
      culture was 275.73 pg/ml. We observed a 2.5-fold induction of VEGF in EACC after 
      72 h, which started with 1.5-fold baseline VEGF concentrations of normal 
      keratinocytes. Our analysis showed that, in the EACC culture, VEGF was elevated 
      after treatment with HGF/SF. HGF/SF appears to activate cellular pathways 
      inducing release of VEGF. After purification, no fibroblasts were present in our 
      EACC culture so as to exclude possible paracrine effects by fibroblasts.
FAU - Naim, Ramin
AU  - Naim R
AD  - Department of Otolaryngology, Head and Neck Surgery, University Hospital 
      Mannheim, D-68135 Mannheim, Germany. ramin.naim@hno.ma.uni-heidelberg.de
FAU - Sadick, Haneen
AU  - Sadick H
FAU - Bayerl, Christiane
AU  - Bayerl C
FAU - Riedel, Frank
AU  - Riedel F
FAU - Schafer, Carsten
AU  - Schafer C
FAU - Bran, Gregor
AU  - Bran G
FAU - Hormann, Karl
AU  - Hormann K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Cells, Cultured
MH  - Cholesteatoma/*metabolism/*pathology
MH  - Ear Canal/*drug effects/*metabolism/pathology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Keratinocytes
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2004/12/08 09:00
MHDA- 2005/05/17 09:00
CRDT- 2004/12/08 09:00
PHST- 2004/12/08 09:00 [pubmed]
PHST- 2005/05/17 09:00 [medline]
PHST- 2004/12/08 09:00 [entrez]
PST - ppublish
SO  - Int J Mol Med. 2005 Jan;15(1):67-71.